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Well-Based Antibody Arrays.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2237, pp. 11-38. - Publication Year :
- 2021
-
Abstract
- Multiplex immunoassays are important tools in basic research and diagnostics. The ability to accurately quantify the presence of several antigens within an individual sample all at once has been useful in developing a proteomics view of biology. This in turn has enabled the development of disease-associated immunodiagnostic panels for better prognosis and well-being. Moreover, it is well understood that such multiplexing approaches lend themselves to automation, thereby reducing labor while providing the ability to dramatically conserve both reagent and sample all of which will reduce the cost per test. Here we describe various methods to create and use multiplex immunoassays in the wells of microtiter plates or similar formats.
- Subjects :
- Animals
Automation, Laboratory methods
Automation, Laboratory standards
Humans
Immunoenzyme Techniques methods
Immunoenzyme Techniques standards
Immunologic Tests economics
Immunologic Tests standards
Protein Array Analysis standards
Sensitivity and Specificity
Immunologic Tests methods
Protein Array Analysis methods
Subjects
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 2237
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 33237405
- Full Text :
- https://doi.org/10.1007/978-1-0716-1064-0_2