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FastFung: A novel medium for the culture and isolation of fastidious fungal species from clinical samples.
- Source :
-
Journal of microbiological methods [J Microbiol Methods] 2021 Jan; Vol. 180, pp. 106108. Date of Electronic Publication: 2020 Nov 21. - Publication Year :
- 2021
-
Abstract
- We developed a novel culture medium, referred to FastFung medium as suitable for the culture of clinical fungi, including fastidious ones, for both research and diagnostic studies. It is based on Schædler agar supplemented with many essential components for the growth of fastidious fungi. It also contains selective antibacterial agents for the inhibition of contaminant bacteria growth. In this preliminary study, the FastFung medium was compared to the gold standard Sabouraud medium for 98 fungal and 20 bacterial strains. The fungal strain positive culture rate was 100% vs. 95% and the bacterial strain inhibition was 100% vs. 20%, for the FastFung and Sabouraud media, respectively. When compared to the Sabouraud medium on 120 clinical samples, the FastFung medium displayed both a higher fungal colonies count, and a lower culture contamination rate. Storage at 4 °C for 4 weeks did not alter the FastFung culture medium performances for the six isolates of Candida, Cryptococcus, and Penicillium tested. These encouraging results suggest future development of using the FastFung medium in clinical mycology and in mycobiome characterization. Further prospective evaluation aiming at assessing whether implementing the FastFung medium in the routine workflow simplifies and strengthen fungal isolation capacities in the clinical laboratory is warranted.<br /> (Copyright © 2020 Elsevier B.V. All rights reserved.)
- Subjects :
- Agar
Bacteria isolation & purification
Candida isolation & purification
Clinical Laboratory Techniques methods
Cryptococcus isolation & purification
Genes, rRNA genetics
Malassezia
Mycobiome
Mycoses diagnosis
Penicillium isolation & purification
Culture Media chemistry
Fungi growth & development
Fungi isolation & purification
Mycology methods
Subjects
Details
- Language :
- English
- ISSN :
- 1872-8359
- Volume :
- 180
- Database :
- MEDLINE
- Journal :
- Journal of microbiological methods
- Publication Type :
- Academic Journal
- Accession number :
- 33232796
- Full Text :
- https://doi.org/10.1016/j.mimet.2020.106108