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Connection between the Gut Microbiome, Systemic Inflammation, Gut Permeability and FOXP3 Expression in Patients with Primary Sjögren's Syndrome.
- Source :
-
International journal of molecular sciences [Int J Mol Sci] 2020 Nov 19; Vol. 21 (22). Date of Electronic Publication: 2020 Nov 19. - Publication Year :
- 2020
-
Abstract
- The aims of this study were to explore intestinal microbial composition and functionality in primary Sjögren's syndrome (pSS) and to relate these findings to inflammation, permeability and the transcription factor Forkhead box protein P3 (FOXP3) gene expression in peripheral blood. The study included 19 pSS patients and 19 healthy controls matched for age, sex, and body mass index. Fecal bacterial DNA was extracted and analyzed by 16S rRNA sequencing using an Ion S5 platform followed by a bioinformatics analysis using Quantitative Insights into Microbial Ecology (QIIME II) and Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt). Our data suggest that the gut microbiota of pSS patients differs at both the taxonomic and functional levels with respect to healthy controls. The gut microbiota profile of our pSS patients was characterized by a lower diversity and richness and with Bacteroidetes dominating at the phylum level. The pSS patients had less beneficial or commensal butyrate-producing bacteria and a higher proportion of opportunistic pathogens with proinflammatory activity, which may impair intestinal barrier function and therefore contribute to inflammatory processes associated with pSS by increasing the production of proinflammatory cytokines and decreasing the release of the anti-inflammatory cytokine IL-10 and the peripheral FOXP3 mRNA expression, implicated in the development and function of regulatory T cells (Treg) cells. Further studies are needed to better understand the real impact of dysbiosis on the course of pSS and to conceive preventive or therapeutic strategies to counteract microbiome-driven inflammation.
- Subjects :
- Actinobacteria classification
Actinobacteria genetics
Actinobacteria isolation & purification
Adolescent
Adult
Aged
Bacteroides classification
Bacteroides genetics
Bacteroides isolation & purification
Body Mass Index
Case-Control Studies
Dysbiosis genetics
Dysbiosis immunology
Dysbiosis pathology
Feces microbiology
Female
Firmicutes classification
Firmicutes genetics
Firmicutes isolation & purification
Forkhead Transcription Factors genetics
Genetic Variation
Humans
Inflammation
Interleukin-10 genetics
Interleukin-10 immunology
Intestines immunology
Middle Aged
Permeability
Proteobacteria classification
Proteobacteria genetics
Proteobacteria isolation & purification
RNA, Ribosomal, 16S genetics
Sjogren's Syndrome genetics
Sjogren's Syndrome immunology
Sjogren's Syndrome pathology
T-Lymphocytes, Regulatory microbiology
Dysbiosis microbiology
Forkhead Transcription Factors immunology
Gastrointestinal Microbiome immunology
Intestines microbiology
Sjogren's Syndrome microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1422-0067
- Volume :
- 21
- Issue :
- 22
- Database :
- MEDLINE
- Journal :
- International journal of molecular sciences
- Publication Type :
- Academic Journal
- Accession number :
- 33228011
- Full Text :
- https://doi.org/10.3390/ijms21228733