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Determination of carcinoembryonic antigen as a tumor marker using a novel graphene-based label-free electrochemical immunosensor.

Authors :
Jozghorbani M
Fathi M
Kazemi SH
Alinejadian N
Source :
Analytical biochemistry [Anal Biochem] 2021 Jan 15; Vol. 613, pp. 114017. Date of Electronic Publication: 2020 Nov 17.
Publication Year :
2021

Abstract

In this work, a simple label-free electrochemical immunosensor for the detection of carcinoembryonic antigen (CEA) was developed. At first, the GC electrode was coated with partially reduced graphene oxide (rGO) to form a platform to bind the antibody. After activating the carboxyl groups of rGO through the EDC/NHS linker, the electrode surface was covered with the antibody. Then, the electrochemical behavior of the antibody-modified electrode and the parameters of the interactions of antibody-antigen immune complexes were investigated using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). This immune-complex layer was found to attenuate the electrochemical current which can be used as a good signal to determine the antigen concentration. The proposed immunosensor exhibited a good amperometric response to CEA within a concentration range of 0.1-5 ng mL <superscript>-1</superscript> with a detection limit of 0.05 ng ml <superscript>-1</superscript> . Furthermore, the developed method was evaluated for the detection of CEA in the real sample (human blood serum), and the results were comparable with the reference values obtained by the standard enzyme-linked immunosorbent assay (ELISA). Our findings suggest the present immunosensor as a good candidate for application in clinical screening.<br /> (Copyright © 2020 Elsevier Inc. All rights reserved.)

Details

Language :
English
ISSN :
1096-0309
Volume :
613
Database :
MEDLINE
Journal :
Analytical biochemistry
Publication Type :
Academic Journal
Accession number :
33212021
Full Text :
https://doi.org/10.1016/j.ab.2020.114017