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Expression of SARS-CoV-2 Entry Factors in the Pancreas of Normal Organ Donors and Individuals with COVID-19.
- Source :
-
Cell metabolism [Cell Metab] 2020 Dec 01; Vol. 32 (6), pp. 1041-1051.e6. Date of Electronic Publication: 2020 Nov 13. - Publication Year :
- 2020
-
Abstract
- Diabetes is associated with increased mortality from severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). Given literature suggesting a potential association between SARS-CoV-2 infection and diabetes induction, we examined pancreatic expression of angiotensin-converting enzyme 2 (ACE2), the key entry factor for SARS-CoV-2 infection. Specifically, we analyzed five public scRNA-seq pancreas datasets and performed fluorescence in situ hybridization, western blotting, and immunolocalization for ACE2 with extensive reagent validation on normal human pancreatic tissues across the lifespan, as well as those from coronavirus disease 2019 (COVID-19) cases. These in silico and ex vivo analyses demonstrated prominent expression of ACE2 in pancreatic ductal epithelium and microvasculature, but we found rare endocrine cell expression at the mRNA level. Pancreata from individuals with COVID-19 demonstrated multiple thrombotic lesions with SARS-CoV-2 nucleocapsid protein expression that was primarily limited to ducts. These results suggest SARS-CoV-2 infection of pancreatic endocrine cells, via ACE2, is an unlikely central pathogenic feature of COVID-19-related diabetes.<br />Competing Interests: Declaration of Interests The authors declare no competing interests.<br /> (Copyright © 2020 Elsevier Inc. All rights reserved.)
- Subjects :
- Angiotensin-Converting Enzyme 2 analysis
Angiotensin-Converting Enzyme 2 metabolism
COVID-19 complications
COVID-19 metabolism
Diabetes Mellitus, Type 2 complications
Diabetes Mellitus, Type 2 genetics
Diabetes Mellitus, Type 2 metabolism
Gene Expression
Humans
Pancreas blood supply
Serine Endopeptidases analysis
Serine Endopeptidases genetics
Serine Endopeptidases metabolism
Tissue Donors
Angiotensin-Converting Enzyme 2 genetics
COVID-19 genetics
Pancreas metabolism
SARS-CoV-2 physiology
Virus Internalization
Subjects
Details
- Language :
- English
- ISSN :
- 1932-7420
- Volume :
- 32
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Cell metabolism
- Publication Type :
- Academic Journal
- Accession number :
- 33207244
- Full Text :
- https://doi.org/10.1016/j.cmet.2020.11.005