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In Vivo Effect of Resveratrol-Loaded Solid Lipid Nanoparticles to Relieve Physical Fatigue for Sports Nutrition Supplements.
- Source :
-
Molecules (Basel, Switzerland) [Molecules] 2020 Nov 13; Vol. 25 (22). Date of Electronic Publication: 2020 Nov 13. - Publication Year :
- 2020
-
Abstract
- Resveratrol (RSV) is a natural flavonoid polyphenol compound extracted from the plants which shows various biological activities. However, the clinical application of RSV is limited by its poor aqueous solubility, rapid metabolism and poor bioavailability. In this study, resveratrol-loaded solid lipid nanoparticles (RSV- SLNs) was design as a nano-antioxidant against the physical fatigue. The resultant RSV-SLNs were characterized by photon correlation spectroscopy (PCS), transmission electron micrographs (TEM), zeta potential, differential scanning calorimetry (DSC) and Raman spectroscopy pattern. Furthermore, the in vivo anti-fatigue effect assays showed that RSV-SLNs prolonged the mice exhausted time and running distance. The biochemical parameters of blood related to fatigue suggested that RSV-SLNs have potential applications to improve the antioxidant defense of the mice after extensive exercise and confer anti-fatigue capability. Furthermore, the molecular mechanisms of antioxidant by RSV-SLNs supplementation was investigated through the analysis of silent information regulator 2 homolog 1 (SIRT1) protein expression, which demonstrated that it could downregulate the expression of SIRT1 and increase autophagy markers, microtubule-associated protein 1 light chain 3-II (LC3-II) and sequestosome-1 (SQSTM1/p62). These results reveal that the RSV-SLNs may have great potential used as a novel anti-fatigue sports nutritional supplement.
- Subjects :
- Animals
Antioxidants chemistry
Biological Availability
Calorimetry, Differential Scanning
Drug Carriers chemistry
Lipid Peroxidation
Male
Mice
Mice, Inbred C57BL
Microscopy, Electron, Transmission
Microtubule-Associated Proteins metabolism
Particle Size
Sequestosome-1 Protein metabolism
Sirtuin 1 metabolism
Solubility
Spectrum Analysis, Raman
Sports Nutritional Physiological Phenomena
Dietary Supplements
Fatigue prevention & control
Lipids chemistry
Nanoparticles chemistry
Physical Conditioning, Animal
Resveratrol chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1420-3049
- Volume :
- 25
- Issue :
- 22
- Database :
- MEDLINE
- Journal :
- Molecules (Basel, Switzerland)
- Publication Type :
- Academic Journal
- Accession number :
- 33202918
- Full Text :
- https://doi.org/10.3390/molecules25225302