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Probing Transcriptome-Wide RNA Structural Changes Dependent on the DEAD-box Helicase Dbp2.
Probing Transcriptome-Wide RNA Structural Changes Dependent on the DEAD-box Helicase Dbp2.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2209, pp. 287-305. - Publication Year :
- 2021
-
Abstract
- RNA helicases function in all aspects of RNA biology mainly through remodeling structures of RNA and RNA-protein (RNP) complexes. Among them, DEAD-box proteins form the largest family in eukaryotes and have been shown to remodel RNA/RNP structures and clamping of RNA-binding proteins, both in vitro and in vivo. Nevertheless, for the majority of these enzymes, it is largely unclear what RNAs are targeted and where they modulate RNA/RNP structures to promote RNA metabolism. Several methods have been developed to probe secondary and tertiary structures of specific transcripts or whole transcriptomes in vivo. In this chapter, we describe a protocol for identification of RNA structural changes that are dependent on a Saccharomyces cerevisiae DEAD-box helicase Dbp2. Experiments detailed here can be adapted to the study of other RNA helicases and identification of putative remodeling targets in vivo.
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 2209
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 33201476
- Full Text :
- https://doi.org/10.1007/978-1-0716-0935-4_18