Back to Search
Start Over
Campylobacter jejuni 11168H Exposed to Penicillin Forms Persister Cells and Cells With Altered Redox Protein Activity.
- Source :
-
Frontiers in cellular and infection microbiology [Front Cell Infect Microbiol] 2020 Oct 19; Vol. 10, pp. 565975. Date of Electronic Publication: 2020 Oct 19 (Print Publication: 2020). - Publication Year :
- 2020
-
Abstract
- The formation of persister cells is one mechanism by which bacteria can survive exposure to environmental stresses. We show that Campylobacter jejuni 11168H forms persister cells at a frequency of 10 <superscript>-3</superscript> after exposure to 100 × MIC of penicillin G for 24 h. Staining the cell population with a redox sensitive fluorescent dye revealed that penicillin G treatment resulted in the appearance of a population of cells with increased fluorescence. We present evidence, to show this could be a consequence of increased redox protein activity in, or associated with, the electron transport chain. These data suggest that a population of penicillin G treated C. jejuni cells could undergo a remodeling of the electron transport chain in order to moderate membrane hyperpolarization and intracellular alkalization; thus reducing the antibiotic efficacy and potentially assisting in persister cell formation.<br /> (Copyright © 2020 Morcrette, Kovacs-Simon, Tennant, Love, Wagley, Yang, Studholme, Soyer, Champion, Butler and Titball.)
Details
- Language :
- English
- ISSN :
- 2235-2988
- Volume :
- 10
- Database :
- MEDLINE
- Journal :
- Frontiers in cellular and infection microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 33194805
- Full Text :
- https://doi.org/10.3389/fcimb.2020.565975