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Beneficial effects resulting from oral administration of Escherichia coli Nissle 1917 on a chronic colitis model.
- Source :
-
Beneficial microbes [Benef Microbes] 2020 Dec 02; Vol. 11 (8), pp. 779-790. Date of Electronic Publication: 2020 Nov 16. - Publication Year :
- 2020
-
Abstract
- Inflammatory bowel diseases (IBD) are chronic processes involving a deregulated immune response against intestinal microbiota in genetically susceptible individuals. Ulcerative colitis (UC) is an IBD restricted to colonic mucosa and its chronicity is a predisposing factor for colorectal cancer (CRC). Probiotics have been investigated as an adjuvant treatment for UC, and Escherichia coli Nissle 1917 (EcN) was the focus of our investigation. The aim of this study was to investigate the preventive effect of the EcN probiotic in an experimental model of chronic colitis in germ-free (GF) and conventional (CV) mice. CV female mice were used for clinical, immunological and permeability experiments. GF mice were used for a faecal microbiota transplantation assay. To induce colitis, three cycles of 3.0% dextran sulphate sodium (DSS) were administered to the animals. For probiotic treatment, the mice received a daily intragastric gavage of 9.0 log <subscript>10</subscript> cfu of EcN, beginning 10 days before colitis induction and continuing until the end of the experiment. EcN presented beneficial effects when administered preventively. Daily Disease Activity Index (DAI) evolution demonstrated significant difference in remission periods after the first two DSS cycles and during the third one. Reduction in bacterial translocation after probiotic treatment indicated protection of the intestinal barrier. Associated with mucosal preservation, restoration of secretory immunoglobulin A levels and reduction of interleukin (IL)-5, IL-13, tumour necrosis factor and interferon-γ levels were observed in EcN treatment. Finally, when microbiota modification was verified, 16S rRNA-based compositional analysis showed variation of intestinal microbiota between the control and colitis groups. After faecal transplantation using GF mice, it was observed that EcN treatment in CV mice might result in modulated intestinal microbiota. This was observed indirectly in the reduced daily DAI, when colitis was compared with treated group. In conclusion, EcN presented beneficial effects in this model, suggesting its usefulness for treating UC.
- Subjects :
- Animals
Colon pathology
Dextran Sulfate toxicity
Disease Models, Animal
Escherichia coli classification
Female
Gastrointestinal Microbiome physiology
Germ-Free Life
Immunoglobulin A analysis
Interferon-gamma blood
Interleukin-13 blood
Interleukin-5 blood
Intestinal Mucosa pathology
Mice
RNA, Ribosomal, 16S genetics
Tumor Necrosis Factor-alpha blood
Colitis, Ulcerative prevention & control
Escherichia coli physiology
Fecal Microbiota Transplantation
Intestinal Mucosa microbiology
Probiotics pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1876-2891
- Volume :
- 11
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Beneficial microbes
- Publication Type :
- Academic Journal
- Accession number :
- 33191778
- Full Text :
- https://doi.org/10.3920/BM2020.0045