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Extraction and characterization of fungal chitin nanofibers from Mucor indicus cultured in optimized medium conditions.
- Source :
-
International journal of biological macromolecules [Int J Biol Macromol] 2021 Jan 15; Vol. 167, pp. 1126-1134. Date of Electronic Publication: 2020 Nov 11. - Publication Year :
- 2021
-
Abstract
- Chitin nanofibers (ChNFs) were extracted from Mucor indicus by a purification method followed by a mechanical treatment, cultivated under obtained optimum culture medium conditions to improve fungal chitin production rate. A semi synthetic media containing 50 g/l glucose was used for cultivation of the fungus in shake flasks. The cell wall analysis showed that N-acetyl glucosamine (GlcNAc) content, which is an indication of chitin content, was maximum in presence of 2.5 g/l H <subscript>3</subscript> PO <subscript>4</subscript> , 2.5 g/l of NaOH, 1 g/l of yeast extract, 1 mg/l of plant hormones, and 1 ml/l of trace metals. The chemical characterizations demonstrated that the isolated fibers had a degree of deacetylation lower than of 10%, and were phosphate free. The FTIR results revealed successful removal of different materials during the purification step. The FE-SEM of fibrillated chitin illustrated an average diameter of 28 nm. Finally, X-ray diffraction results showed that the crystallinity index of nanofibers was 82%.<br /> (Copyright © 2020 Elsevier B.V. All rights reserved.)
- Subjects :
- Biomass
Chemical Fractionation methods
Chitin isolation & purification
Chitosan chemistry
Culture Media analysis
Culture Media, Conditioned analysis
Culture Media, Conditioned chemistry
Phosphates chemistry
Spectroscopy, Fourier Transform Infrared
X-Ray Diffraction
Chitin chemistry
Culture Media chemistry
Fungal Polysaccharides chemistry
Mucor chemistry
Nanofibers chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0003
- Volume :
- 167
- Database :
- MEDLINE
- Journal :
- International journal of biological macromolecules
- Publication Type :
- Academic Journal
- Accession number :
- 33188816
- Full Text :
- https://doi.org/10.1016/j.ijbiomac.2020.11.066