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Plasmodium falciparum maturation across the intra-erythrocytic cycle shifts the soft glassy viscoelastic properties of red blood cells from a liquid-like towards a solid-like behavior.
- Source :
-
Experimental cell research [Exp Cell Res] 2020 Dec 15; Vol. 397 (2), pp. 112370. Date of Electronic Publication: 2020 Nov 11. - Publication Year :
- 2020
-
Abstract
- The mechanical properties of erythrocytes have been investigated by different techniques. However, there are few reports on how the viscoelasticity of these cells varies during malaria disease. Here, we quantitatively map the viscoelastic properties of Plasmodium falciparum-parasitized human erythrocytes. We apply new methodologies based on optical tweezers to measure the viscoelastic properties and defocusing microscopy to measure the erythrocyte height profile, the overall cell volume, and its form factor, a crucial parameter to convert the complex elastic constant into complex shear modulus. The storage and loss shear moduli are obtained for each stage of parasite maturation inside red blood cells, while the former increase, the latter decrease. Employing a soft glassy rheology model, we obtain the power-law exponent for the storage and loss shear moduli, characterizing the soft glassy features of red blood cells in each parasite maturation stage. Ring forms present a liquid-like behavior, with a slightly lower power-law exponent than healthy erythrocytes, whereas trophozoite and schizont stages exhibit increasingly solid-like behaviors. Finally, the surface elastic shear moduli, low-frequency surface viscosities, and shape recovery relaxation times all increase not only in a stage-dependent manner but also when compared to healthy red blood cells. Overall, the results call attention to the soft glassy characteristics of Plasmodium falciparum-parasitized erythrocyte membrane and may provide a basis for future studies to better understand malaria disease from a mechanobiological perspective.<br /> (Copyright © 2020 Elsevier Inc. All rights reserved.)
- Subjects :
- Blood Viscosity
Erythrocyte Membrane parasitology
Erythrocytes parasitology
Erythrocytes, Abnormal parasitology
Humans
Malaria parasitology
Plasmodium falciparum pathogenicity
Rheology
Elastic Modulus
Erythrocyte Membrane pathology
Erythrocytes pathology
Erythrocytes, Abnormal pathology
Malaria blood
Plasmodium falciparum growth & development
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2422
- Volume :
- 397
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Experimental cell research
- Publication Type :
- Academic Journal
- Accession number :
- 33186602
- Full Text :
- https://doi.org/10.1016/j.yexcr.2020.112370