Nocardioides convexus sp. nov. and Nocardioides anomalus sp. nov., isolated from soil and mineral water.

Two bacterial strains designated as W3-2-3 ^T and HKS04 ^T were isolated from mineral water and a soil sample, respectively, in the Republic of Korea. The 16S rRNA genes of the two strains shared a sequence similarity of 93.5 %. Phylogenetic analysis based on 16S rRNA gene sequences showed that strains W3-2-3 ^T and HKS04 ^T formed a distinct lineage within the genus Nocardioides of the family Nocardioidaceae (order Propionibacteriales ). The closely related species of strain W3-2-3 ^T were Nocardioides albidus (98.9 %), Nocardioides caeni (98.8 %), Nocardioides kongjuensis (98.6 %), Nocardioides aromaticivorans (98.5 %), Nocardioides nitrophenolicus (98.4 %), Nocardioides flava (98.2 %) and Nocardioides ginsengisoli (98.1 %). The closest species of strain HKS04 ^T was Nocardioides halotolerans (98.7 %). The genome sizes of strains W3-2-3 ^T and HKS04 ^T were 4741198 and 5 120341 bp, respectively. The genomic DNA G+C contents of strains W3-2-3 ^T and HKS04 ^T were 73.3 and 72.1 mol%, respectively. The main fatty acids of strain W3-2-3 ^T were C _17:1 ω 6 c and iso-C _16:0 and those of strain HKS04 ^T were iso-C _16:0 and iso-C _16:0 H. The main polar lipids of both strains were diphosphatidylglycerol and phosphatidylglycerol and the predominant respiratory quinone was MK-8(H ₄ ), supporting the affiliation of these strains with the genus Nocardioides . Based on the results of biochemical, chemotaxonomic and phylogenetic analyses, two novel species, Nocardioides convexus W3-2-3 ^T (KACC 21211 ^T =LMG 31251 ^T ) and Nocardioides anomalus HKS04 ^T (KACC 18879 ^T =LMG 31249 ^T ), are proposed.