Back to Search
Start Over
External and internal EGFR-activating signals drive mammary epithelial cells proliferation and viability.
- Source :
-
Molecular and cellular endocrinology [Mol Cell Endocrinol] 2021 Jan 15; Vol. 520, pp. 111081. Date of Electronic Publication: 2020 Nov 09. - Publication Year :
- 2021
-
Abstract
- During puberty, the mammary gland undergoes an intense growth, dependent on the interplay between the Epidermal Growth Factor Receptor (EGFR) in the stroma and different mammary epithelial receptors. We hypothesize that EGFR expressed in the mammary epithelium also has a role in puberty and the epithelial cells can self-sustain by EGFR-mediated autocrine signaling. We adopted mammary cell lines from different species, as in vitro model for the epithelium, and we observed that EGFR-signaling positively affects their survival and proliferation. Once deprived of external growth factors, mammary cells still showed strong Erk 1/2 phosphorylation, abolished upon EGFR inhibition, coupled with a further reduction in survival and proliferation. Based on gene expression analysis, three EGFR-ligands (AREG, EREG and HBEGF) are likely to mediate this autocrine signaling. In conclusion, internal EGFR-activating signals sustain mammary epithelial cell proliferation and survival in vitro.<br /> (Copyright © 2020 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Autocrine Communication
Cattle
Cell Cycle
Cell Line
Cell Proliferation
Cell Survival
ErbB Receptors antagonists & inhibitors
Extracellular Signal-Regulated MAP Kinases metabolism
Female
Humans
Intercellular Signaling Peptides and Proteins deficiency
Keratin-14 metabolism
Keratin-18 metabolism
Ligands
Mice
Receptor, ErbB-2 metabolism
Species Specificity
Epithelial Cells cytology
Epithelial Cells metabolism
ErbB Receptors metabolism
Mammary Glands, Animal cytology
Mammary Glands, Human cytology
Signal Transduction
Subjects
Details
- Language :
- English
- ISSN :
- 1872-8057
- Volume :
- 520
- Database :
- MEDLINE
- Journal :
- Molecular and cellular endocrinology
- Publication Type :
- Academic Journal
- Accession number :
- 33181234
- Full Text :
- https://doi.org/10.1016/j.mce.2020.111081