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The catalytic activity of the translation termination factor methyltransferase Mtq2-Trm112 complex is required for large ribosomal subunit biogenesis.
- Source :
-
Nucleic acids research [Nucleic Acids Res] 2020 Dec 02; Vol. 48 (21), pp. 12310-12325. - Publication Year :
- 2020
-
Abstract
- The Mtq2-Trm112 methyltransferase modifies the eukaryotic translation termination factor eRF1 on the glutamine side chain of a universally conserved GGQ motif that is essential for release of newly synthesized peptides. Although this modification is found in the three domains of life, its exact role in eukaryotes remains unknown. As the deletion of MTQ2 leads to severe growth impairment in yeast, we have investigated its role further and tested its putative involvement in ribosome biogenesis. We found that Mtq2 is associated with nuclear 60S subunit precursors, and we demonstrate that its catalytic activity is required for nucleolar release of pre-60S and for efficient production of mature 5.8S and 25S rRNAs. Thus, we identify Mtq2 as a novel ribosome assembly factor important for large ribosomal subunit formation. We propose that Mtq2-Trm112 might modify eRF1 in the nucleus as part of a quality control mechanism aimed at proof-reading the peptidyl transferase center, where it will subsequently bind during translation termination.<br /> (© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.)
- Subjects :
- Binding Sites
Biocatalysis
Cloning, Molecular
Crystallography, X-Ray
Escherichia coli genetics
Escherichia coli metabolism
Genetic Vectors chemistry
Genetic Vectors metabolism
Methyltransferases chemistry
Methyltransferases metabolism
Models, Molecular
Peptide Chain Termination, Translational
Peptide Termination Factors metabolism
Protein Binding
Protein Conformation, alpha-Helical
Protein Conformation, beta-Strand
Protein Interaction Domains and Motifs
RNA, Ribosomal biosynthesis
RNA, Ribosomal genetics
RNA, Ribosomal, 5.8S biosynthesis
RNA, Ribosomal, 5.8S genetics
Recombinant Proteins chemistry
Recombinant Proteins genetics
Recombinant Proteins metabolism
Ribosome Subunits, Large, Eukaryotic metabolism
Saccharomyces cerevisiae metabolism
Saccharomyces cerevisiae Proteins chemistry
Saccharomyces cerevisiae Proteins metabolism
Substrate Specificity
tRNA Methyltransferases chemistry
tRNA Methyltransferases metabolism
Gene Expression Regulation, Fungal
Methyltransferases genetics
Organelle Biogenesis
Peptide Termination Factors genetics
Ribosome Subunits, Large, Eukaryotic genetics
Saccharomyces cerevisiae genetics
Saccharomyces cerevisiae Proteins genetics
tRNA Methyltransferases genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1362-4962
- Volume :
- 48
- Issue :
- 21
- Database :
- MEDLINE
- Journal :
- Nucleic acids research
- Publication Type :
- Academic Journal
- Accession number :
- 33166396
- Full Text :
- https://doi.org/10.1093/nar/gkaa972