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Roles of a small GTPase Sar1 in ecdysteroid signaling and immune response of red swamp crayfish Procambarus clarkii.
- Source :
-
International journal of biological macromolecules [Int J Biol Macromol] 2021 Jan 01; Vol. 166, pp. 550-556. Date of Electronic Publication: 2020 Nov 01. - Publication Year :
- 2021
-
Abstract
- Secretion-associated and ras-related protein 1 (Sar1) is a small GTPase that plays an important role in the transport of protein coated with coat protein complex II vesicles. However, its alternative roles in the biological processes of Procambarus clarkii remain unclear. Here, a sar1 gene (named as Pc-sar1) with an open reading frame of 582 bp from P. clarkii was identified. Pc-sar1 was expressed in all examined tissues with highest expression levels in muscle, which was determined by real-time PCR and western blotting. After the induction of lipopolysaccharide (LPS) and polycytidylic acid (Poly I: C), the transcriptional levels of Pc-sar1 differed in hepatopancreas, gill, muscle and intestine. In contrast, the expression of Pc-sar1 was upregulated by 20-hydroxyecdysone in these four tissues. In addition, the RNA interference of Pc-sar1 significantly affected the expression levels of immune and hormone-related genes. These results indicate that Pc-sar1 is involved in the innate immune response and ecdysteroid signaling pathway.<br />Competing Interests: Declaration of competing interest The authors have no conflict of interest to declare.<br /> (Copyright © 2018 Elsevier B.V. All rights reserved.)
- Subjects :
- Amino Acid Sequence
Animals
Astacoidea drug effects
Astacoidea genetics
Ecdysterone pharmacology
Gene Expression Regulation drug effects
Lipopolysaccharides pharmacology
Monomeric GTP-Binding Proteins chemistry
Monomeric GTP-Binding Proteins isolation & purification
Phylogeny
Poly I-C pharmacology
RNA Interference
Tissue Distribution drug effects
Astacoidea enzymology
Astacoidea immunology
Ecdysteroids metabolism
Monomeric GTP-Binding Proteins metabolism
Signal Transduction
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0003
- Volume :
- 166
- Database :
- MEDLINE
- Journal :
- International journal of biological macromolecules
- Publication Type :
- Academic Journal
- Accession number :
- 33137382
- Full Text :
- https://doi.org/10.1016/j.ijbiomac.2020.10.212