Back to Search
Start Over
ZBTB gene expression in HIV patients: a possible new molecular mechanism of viral control.
- Source :
-
Archives of virology [Arch Virol] 2021 Jan; Vol. 166 (1), pp. 167-178. Date of Electronic Publication: 2020 Nov 01. - Publication Year :
- 2021
-
Abstract
- HIV infects its target cell and integrates into its genome as an essential step in its replication cycle. Proviral DNA is also subjected to the same transcriptional regulation as the host cell genome by its own transcriptional factors, with activating or repressive activity. There is a clear interaction between the presence of transcriptional repressors and a decrease in the rate of HIV replication, promoting gene silencing in infected cells, which serve as viral reservoirs. This represents a major obstacle for HIV eradication. The ZBTB gene family comprises 49 genes that encode transcription factors that have a repressor function in differentiation and development of cells of the lymphopoietic lineage, including the main target cells of HIV, CD4 <superscript>+</superscript> T cells. In this cross-sectional study, we evaluated the expression profile of ZBTB genes in CD4 <superscript>+</superscript> T cells of HIV-positive individuals with different levels of infection control. We found upregulation of gene expression of ZBTB4 (p < 0.01), ZBTB7B (p < 0.001), and ZBTB38 (p < 0.05) and downregulation of ZBTB16 (p < 0.01) in HIV-positive patients compared to HIV-negative individuals. Interestingly, in a deeper analysis, we observed that elite controllers had the highest levels of expression of the ZBTB38, ZBTB2, HIC1, ZBTB7A, ZBTB7B (ThPOK) and ZBTB4 genes, showing 2.56- to 7.60-fold upregulation compare to the ART-naïve group. These results suggest a possible contribution of these ZBTB transcriptional repressors in HIV-positive patients and a possible new molecular mechanism of viral control.
- Subjects :
- Adult
CD4-Positive T-Lymphocytes virology
Cell Line
Female
Gene Expression genetics
Gene Expression Regulation genetics
HIV-1 pathogenicity
Humans
Leukocytes, Mononuclear virology
Male
Virus Latency genetics
Virus Replication genetics
DNA-Binding Proteins genetics
HIV Infections genetics
HIV Infections virology
Transcription Factors genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1432-8798
- Volume :
- 166
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Archives of virology
- Publication Type :
- Academic Journal
- Accession number :
- 33130911
- Full Text :
- https://doi.org/10.1007/s00705-020-04854-6