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Development of an in-house capture ELISA: An attempt to detect CagA antigen in sera of Helicobacter pylori infected patients.
- Source :
-
Journal of immunological methods [J Immunol Methods] 2021 Jan; Vol. 488, pp. 112905. Date of Electronic Publication: 2020 Oct 29. - Publication Year :
- 2021
-
Abstract
- The CagA protein one of the key virulence factors of Helicobacter pylori plays an important role in the pathogenesis of peptic ulcer diseases. Unfortunately the cagA gene status can only be determined by PCR while serology is an alternative approach to detect antigens or antibodies. Our aim is to detect the CagA antigen in sera of infected subjects by the development of an in-house capture ELISA test. Gastric antral biopsies and serum samples were collected from 63 patients. PCR was used to determine the cagA status. Our previously developed recombinant CagA protein and monoclonal antibody were used for setting up the capture ELISA test. H. pylori positive [(38 gastritis, 14 duodenal ulcers (DU), 11 gastric ulcer (GU)] patients were determined by PCR. The cagA gene was detected in 21 (55%) of gastritis, 11 (78%) of DU and 7 (60%) of GU patients. The reagents used in setting up the capture ELISA test following optimization displayed high performance. This study showed that our developed in-house capture ELISA has the potential to detect the CagA antigen at very low concentrations even though not detected in our H. pylori infected patients sera but we are also intended to use it in saliva and stool samples.<br /> (Copyright © 2020. Published by Elsevier B.V.)
- Subjects :
- Biomarkers blood
Gastritis blood
Gastritis immunology
Gastritis microbiology
Helicobacter Infections blood
Helicobacter Infections immunology
Helicobacter Infections microbiology
Humans
Peptic Ulcer blood
Peptic Ulcer immunology
Peptic Ulcer microbiology
Predictive Value of Tests
Reproducibility of Results
Antigens, Bacterial blood
Bacterial Proteins blood
Enzyme-Linked Immunosorbent Assay
Gastritis diagnosis
Helicobacter Infections diagnosis
Helicobacter pylori immunology
Peptic Ulcer diagnosis
Serologic Tests
Subjects
Details
- Language :
- English
- ISSN :
- 1872-7905
- Volume :
- 488
- Database :
- MEDLINE
- Journal :
- Journal of immunological methods
- Publication Type :
- Academic Journal
- Accession number :
- 33129887
- Full Text :
- https://doi.org/10.1016/j.jim.2020.112905