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Sheng-Mai Yin exerts anti-inflammatory effects on RAW 264.7 cells and zebrafish.
- Source :
-
Journal of ethnopharmacology [J Ethnopharmacol] 2021 Mar 01; Vol. 267, pp. 113497. Date of Electronic Publication: 2020 Oct 19. - Publication Year :
- 2021
-
Abstract
- Ethnopharmacological Relevance: Sheng-Mai Yin (SMY), a famous traditional Chinese medicine formula, has been commonly used in China for centuries to treat various diseases, such as inflammation-related diseases. However, the anti-inflammatory activity of SMY and its potential mechanisms still have not yet been clearly understood.<br />Aim of the Study: In this study, we aimed to determine the anti-inflammatory effect of SMY and explore its underlying mechanisms both on RAW 264.7 cells and zebrafish.<br />Materials and Methods: The levels of pro-inflammatory cytokines IL-6 and TNF-α secreted by RAW 264.7 cells were measured by ELISA. The protein expressions of IκBα, p-IκBα (Ser32), STAT3 and p-STAT3 (Tyr705) were determined by Western blotting. And the nuclear translocation of NF-κB p65 in LPS-induced RAW 264.7 macrophage cells was detected by confocal microscopy. Moreover, the in vivo anti-inflammatory effect of SMY and its potential mechanisms were further investigated by survival analysis, hematoxylin-eosin staining (H&E), observation of neutrophil migration and quantitative real-time PCR (qRT-PCR) analysis in zebrafish inflammatory models.<br />Results: SMY reduced the release of IL-6 and TNF-α, inhibited the phosphorylation of IκBα and STAT3 as well as the nuclear translocation of NF-κB p65 in LPS-induced RAW 264.7 cells. Furthermore, the increased survival, decreased infiltration of inflammatory cells and the attenuated migration of neutrophils together suggested the in vivo anti-inflammatory effects of SMY. More importantly, SMY reduced the gene expressions of pro-inflammatory cytokines and suppressed LPS-induced up-regulation of NF-κB, IκBα and STAT3 in zebrafish inflammatory models.<br />Conclusion: SMY exerts significant anti-inflammatory effects with a potential mechanism of inhibiting the NF-κB and STAT3 signal pathways. Our findings suggest a scientific rationale of SMY to treat inflammatory diseases in clinic.<br /> (Copyright © 2020. Published by Elsevier B.V.)
- Subjects :
- Animals
Animals, Genetically Modified
Copper Sulfate
Cytokines metabolism
Disease Models, Animal
Drug Combinations
Inflammation chemically induced
Inflammation immunology
Inflammation metabolism
Lipopolysaccharides
Macrophages immunology
Macrophages metabolism
Mice
NF-KappaB Inhibitor alpha metabolism
NF-kappa B metabolism
Neutrophil Infiltration drug effects
Neutrophils immunology
Neutrophils metabolism
RAW 264.7 Cells
STAT3 Transcription Factor metabolism
Signal Transduction
Zebrafish embryology
Zebrafish genetics
Zebrafish Proteins metabolism
Anti-Inflammatory Agents pharmacology
Drugs, Chinese Herbal pharmacology
Inflammation prevention & control
Inflammation Mediators metabolism
Macrophages drug effects
Neutrophils drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1872-7573
- Volume :
- 267
- Database :
- MEDLINE
- Journal :
- Journal of ethnopharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 33091492
- Full Text :
- https://doi.org/10.1016/j.jep.2020.113497