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The role of miR-128-3p through MAPK14 activation in the apoptosis of GC2 spermatocyte cell line following heat stress.
- Source :
-
Andrology [Andrology] 2021 Mar; Vol. 9 (2), pp. 665-672. Date of Electronic Publication: 2020 Nov 05. - Publication Year :
- 2021
-
Abstract
- Background: MicroRNAs play a crucial role in the regulation of spermatogenesis. For example, miR-128-3p expression is known to decrease significantly after testicular hyperthermia, but the regulatory effect of this change on the spermatogenesis damage caused by heat stress remains unclear.<br />Objectives: This study aimed to verify whether the target gene of miR-128-3p is MAPK14, which affects spermatogenic cell proliferation and apoptosis under testicular hyperthermia.<br />Materials and Methods: Mouse testis and GC2 spermatocyte cell line heat stress models were established. miR-128-3p expression before and after heat stress was analyzed by reverse transcription polymerase chain reaction. MAPK14 and p-MAPK14 expression was detected by Western blot, and cell apoptosis was analyzed by Annexin V-FITC/PI. Subsequently, miR-128-3p inhibitors and mimics were used to interfere with spermatocytes before and after heat stress, respectively, for correlation detection.<br />Results: Compared with the control group, the heat stress group showed decreased miR-128-3p expression, increased p-MAPK14 expression, and decreased cell proliferation activity. In the GC2-spd cell line in vitro, miR-128-3p inhibitors were found to upregulate p-MAPK14 expression, reduce cell proliferation activity, and increase apoptosis, consistent with the results obtained in the heat treatment alone. Furthermore, miR-128-3p mimics transfected in the GC2 cells after heat stress reduced p-MAPK14 expression, alleviated the decrease in cell proliferation, and decreased the apoptosis level.<br />Conclusions: The downregulation of miR-128-3p expression plays an important role in spermatogenesis damages after testicular hyperthermia, which is probably attributable to the activation of the MAPK signaling pathway. Downregulated miR-128-3p expression induces the apoptosis and inhibits the proliferation of spermatogenic cells by promoting MAPK14 phosphorylation.<br /> (© 2020 American Society of Andrology and European Academy of Andrology.)
- Subjects :
- Animals
Cell Line
Enzyme Activation genetics
Gene Expression Regulation, Developmental
Heat-Shock Response
Male
Mice
Mice, Inbred ICR
Mitogen-Activated Protein Kinase 14
Spermatocytes enzymology
Spermatogenesis genetics
Testis cytology
Testis enzymology
Apoptosis genetics
MicroRNAs physiology
Spermatocytes physiology
Testis metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2047-2927
- Volume :
- 9
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Andrology
- Publication Type :
- Academic Journal
- Accession number :
- 33089633
- Full Text :
- https://doi.org/10.1111/andr.12923