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Cell morphology maintenance in Bacillus subtilis through balanced peptidoglycan synthesis and hydrolysis.
- Source :
-
Scientific reports [Sci Rep] 2020 Oct 21; Vol. 10 (1), pp. 17910. Date of Electronic Publication: 2020 Oct 21. - Publication Year :
- 2020
-
Abstract
- The peptidoglycan layer is responsible for maintaining bacterial cell shape and permitting cell division. Cell wall growth is facilitated by peptidoglycan synthases and hydrolases and is potentially modulated by components of the central carbon metabolism. In Bacillus subtilis, UgtP synthesises the glucolipid precursor for lipoteichoic acid and has been suggested to function as a metabolic sensor governing cell size. Here we show that ugtP mutant cells have increased levels of cell wall precursors and changes in their peptidoglycan that suggest elevated DL-endopeptidase activity. The additional deletion of lytE, encoding a DL-endopeptidase important for cell elongation, in the ugtP mutant background produced cells with severe shape defects. Interestingly, the ugtP lytE mutant recovered normal rod-shape by acquiring mutations that decreased the expression of the peptidoglycan synthase PBP1. Together our results suggest that cells lacking ugtP must re-adjust the balance between peptidoglycan synthesis and hydrolysis to maintain proper cell morphology.
- Subjects :
- Bacterial Proteins genetics
Bacterial Proteins metabolism
Carbon metabolism
Cell Division
Cell Wall metabolism
Endopeptidases metabolism
Hydrolysis
Lipopolysaccharides metabolism
Mutation
N-Acetylmuramoyl-L-alanine Amidase genetics
N-Acetylmuramoyl-L-alanine Amidase metabolism
Teichoic Acids metabolism
Bacillus subtilis cytology
Bacillus subtilis metabolism
Cell Shape genetics
Cell Shape physiology
Peptidoglycan biosynthesis
Peptidoglycan metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 10
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 33087775
- Full Text :
- https://doi.org/10.1038/s41598-020-74609-5