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Is DAPI assay of cellular nucleic acid reliable in the presence of protein aggregates?
- Source :
-
Chemical communications (Cambridge, England) [Chem Commun (Camb)] 2020 Nov 18; Vol. 56 (89), pp. 13844-13847. Date of Electronic Publication: 2020 Oct 21. - Publication Year :
- 2020
-
Abstract
- DAPI is used extensively to identify and quantify DNA in cellular systems assuming its exclusive staining of nucleic acids. However, the present results show that DAPI has much higher affinity towards protein aggregates than DNA. Thus, the use of DAPI for the nucleic acid assay of cellular systems with protein aggregates may not be very reliable.
Details
- Language :
- English
- ISSN :
- 1364-548X
- Volume :
- 56
- Issue :
- 89
- Database :
- MEDLINE
- Journal :
- Chemical communications (Cambridge, England)
- Publication Type :
- Academic Journal
- Accession number :
- 33084648
- Full Text :
- https://doi.org/10.1039/d0cc04108d