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Tracking elemental changes in an ischemic stroke model with X-ray fluorescence imaging.

Authors :
Pushie MJ
Sylvain NJ
Hou H
Caine S
Hackett MJ
Kelly ME
Source :
Scientific reports [Sci Rep] 2020 Oct 20; Vol. 10 (1), pp. 17868. Date of Electronic Publication: 2020 Oct 20.
Publication Year :
2020

Abstract

Stroke is a leading cause of long-term disability in adults and a leading cause of death in developed nations. The cascade of cellular events and signalling that occur after cerebral ischemia are complex, however, analyzing global element markers of metabolic state affords the means to monitor stroke severity, status of injury, and recovery. These markers provide a multi-parameter method for assessing changes through the post-stroke time course. We employ synchrotron-based elemental mapping to follow elemental changes in the brain at 1 h, 1-, 2-, and 3-days, and at 1-, 2-, 3-, and 4-weeks post-stroke in a photothrombotic stroke model in mice. Our analysis reveals a highly consistent metabolic penumbra that can be readily identified based on the level of dysregulated potassium and other key elements. Maps of elemental distributions are also useful to demarcate events in the cellular response to the inflammatory cascade, including ion dysregulation, recruitment of cells to the lesion, and glial scar formation.

Details

Language :
English
ISSN :
2045-2322
Volume :
10
Issue :
1
Database :
MEDLINE
Journal :
Scientific reports
Publication Type :
Academic Journal
Accession number :
33082455
Full Text :
https://doi.org/10.1038/s41598-020-74698-2