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Fragmentation of the Golgi complex of dopaminergic neurons in human substantia nigra: New cytopathological findings in Parkinson's disease.
- Source :
-
Histology and histopathology [Histol Histopathol] 2021 Jan; Vol. 36 (1), pp. 47-60. Date of Electronic Publication: 2020 Oct 20. - Publication Year :
- 2021
-
Abstract
- Fragmentation of the Golgi ribbon is a common feature of Parkinson´s disease and other neurodegenerative diseases. This alteration could be the consequence of the anterograde and retrograde transport imbalance, α-synuclein aggregates, and/or cytoskeleton alterations. Most information on this process has been obtained from cellular and animal experimental models, and as such, there is little information available on human tissue. If the information on human tissue was available, it may help to understand the cytopathological mechanisms of this disease. In the present study, we analyzed the morphological characteristics of the Golgi complex of dopaminergic neurons in human samples of substantia nigra of control and Parkinson's disease patients. We measured the expression levels of putative molecules involved in Golgi fragmentation, including α-synuclein, tubulin, and Golgi-associated regulatory and structural proteins. We show that, as a consequence of the disease, the Golgi complex is fragmented into small stacks without vesiculation. We found that only a limited number of regulatory proteins are altered. Rab1, a small GTPase regulating endoplasmic reticulum-to-Golgi transport, is the most dramatically affected, being highly overexpressed in the surviving neurons. We found that the SNARE protein syntaxin 5 forms extracellular aggregates resembling the amyloid plaques characteristic of Alzheimer's disease. These findings may help to understand the cytopathology of Parkinson's disease.
- Subjects :
- Adult
Aged
Aged, 80 and over
Animals
Dopamine metabolism
Dopaminergic Neurons metabolism
Female
Golgi Apparatus metabolism
Humans
Immunohistochemistry
Male
Melanins metabolism
Middle Aged
Neurons metabolism
PC12 Cells
Parkinson Disease metabolism
Qa-SNARE Proteins metabolism
Rats
Substantia Nigra metabolism
Tubulin metabolism
alpha-Synuclein metabolism
rab1 GTP-Binding Proteins metabolism
Dopaminergic Neurons pathology
Golgi Apparatus pathology
Parkinson Disease pathology
Substantia Nigra pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1699-5848
- Volume :
- 36
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Histology and histopathology
- Publication Type :
- Academic Journal
- Accession number :
- 33078843
- Full Text :
- https://doi.org/10.14670/HH-18-270