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Nonsteroidal antiinflammatory drugs alter antibiotic susceptibility and expression of virulence-related genes and protein A of Staphylococcus aureus
- Source :
-
Turkish journal of medical sciences [Turk J Med Sci] 2021 Apr 30; Vol. 51 (2), pp. 835-847. Date of Electronic Publication: 2021 Apr 30. - Publication Year :
- 2021
-
Abstract
- Background/aim: Nonsteroidal antiinflammatory drugs (NSAIDs) including diclofenac, naproxen, ibuprofen, acetylsalicylic acid, and acetaminophen have been shown to have antimicrobial effects on various microorganisms. The aim of this study was to investigate the antibacterial effects of NSAIDs on Staphylococcus aureus.<br />Materials and Methods: Susceptibilities of S. aureus strains to NSAIDs with or without antimicrobials (moxifloxacin, vancomycin, ciprofloxacin, clindamycin, and gentamicin) were determined using the microdilution method and disk diffusion test. Expression levels of genes in the presence of drugs were investigated by real-time quantitative RT-PCR (qRT-PCR), and immunoblotting analysis was performed for staphylococcal protein A (SpA).<br />Results: Our results showed that all NSAIDs were active against S. aureus strains with MIC values ranging from 195 μg/mL to 6250 μg/ mL. NSAIDs increased the antibiotic susceptibility of the strains, and diclofenac was found to be more effective than the other drugs. Drugs showed different effects on expression levels of virulence factor and/or regulatory genes. Immunoblotting analysis of SpA protein was mostly in accordance with qRT-PCR results.<br />Conclusion: The regulatory/virulence factor genes and proteins of S. aureus investigated in this study may be reasonable targets for these drugs, and we suggest that the data may contribute to the field of infection control and antimicrobial resistance.<br />Competing Interests: none declared.<br /> (This work is licensed under a Creative Commons Attribution 4.0 International License.)
- Subjects :
- Anti-Bacterial Agents therapeutic use
Anti-Inflammatory Agents, Non-Steroidal therapeutic use
Diclofenac pharmacology
Humans
Microbial Sensitivity Tests
Real-Time Polymerase Chain Reaction
Staphylococcal Infections drug therapy
Staphylococcal Protein A genetics
Staphylococcal Protein A therapeutic use
Staphylococcus aureus genetics
Virulence genetics
Virulence Factors genetics
Anti-Bacterial Agents pharmacology
Anti-Inflammatory Agents, Non-Steroidal pharmacology
Gene Expression drug effects
Staphylococcus aureus drug effects
Virulence drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1303-6165
- Volume :
- 51
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Turkish journal of medical sciences
- Publication Type :
- Academic Journal
- Accession number :
- 33078603
- Full Text :
- https://doi.org/10.3906/sag-2003-60