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Shortwave infrared polymethine fluorophores matched to excitation lasers enable non-invasive, multicolour in vivo imaging in real time.
- Source :
-
Nature chemistry [Nat Chem] 2020 Dec; Vol. 12 (12), pp. 1123-1130. Date of Electronic Publication: 2020 Oct 19. - Publication Year :
- 2020
-
Abstract
- High-resolution, multiplexed experiments are a staple in cellular imaging. Analogous experiments in animals are challenging, however, due to substantial scattering and autofluorescence in tissue at visible (350-700 nm) and near-infrared (700-1,000 nm) wavelengths. Here, we enable real-time, non-invasive multicolour imaging experiments in animals through the design of optical contrast agents for the shortwave infrared (SWIR, 1,000-2,000 nm) region and complementary advances in imaging technologies. We developed tunable, SWIR-emissive flavylium polymethine dyes and established relationships between structure and photophysical properties for this class of bright SWIR contrast agents. In parallel, we designed an imaging system with variable near-infrared/SWIR excitation and single-channel detection, facilitating video-rate multicolour SWIR imaging for optically guided surgery and imaging of awake and moving mice with multiplexed detection. Optimized dyes matched to 980 nm and 1,064 nm lasers, combined with the clinically approved indocyanine green, enabled real-time, three-colour imaging with high temporal and spatial resolutions.
- Subjects :
- Animals
Benzopyrans chemical synthesis
Benzopyrans radiation effects
Contrast Media chemical synthesis
Contrast Media radiation effects
Female
Fluorescent Dyes chemical synthesis
Fluorescent Dyes radiation effects
Infrared Rays
Lasers
Mice, Nude
Optical Imaging instrumentation
Benzopyrans chemistry
Contrast Media chemistry
Fluorescent Dyes chemistry
Optical Imaging methods
Subjects
Details
- Language :
- English
- ISSN :
- 1755-4349
- Volume :
- 12
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Nature chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 33077925
- Full Text :
- https://doi.org/10.1038/s41557-020-00554-5