Back to Search
Start Over
Polyglutamine-Related Aggregates Can Serve as a Potent Antigen Source for Cross-Presentation by Dendritic Cells.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2020 Nov 15; Vol. 205 (10), pp. 2583-2594. Date of Electronic Publication: 2020 Oct 16. - Publication Year :
- 2020
-
Abstract
- Protective MHC class I-dependent immune responses require an overlap between repertoires of proteins directly presented on target cells and cross-presented by professional APC, specifically dendritic cells. How stable proteins that rely on defective ribosomal proteins for direct presentation are captured for cell-to-cell transfer remains enigmatic. In this study, we address this issue using a combination of in vitro (C57BL/6-derived mouse cell lines) and in vivo (C57BL/6 mouse strains) approaches involving stable and unstable versions of OVA model Ags displaying defective ribosomal protein-dependent and -independent Ag presentation, respectively. Apoptosis, but not necrosis, of donor cells was found associated with robust global protein aggregate formation and captured stable proteins permissive for cross-presentation. Potency of aggregates to serve as Ag source was directly demonstrated using polyglutamine-equipped model substrates. Collectively, our data implicate global protein aggregation in apoptotic cells as a mechanism that ensures the overlap between MHC class I epitopes presented directly or cross-presented by APC and demonstrate the unusual ability of dendritic cells to process stable protein aggregates.<br /> (Copyright © 2020 by The American Association of Immunologists, Inc.)
- Subjects :
- Animals
Antigens genetics
Cell Line
Dendritic Cells metabolism
Epitopes immunology
Female
Histocompatibility Antigens Class I immunology
Histocompatibility Antigens Class I metabolism
Mice
Mice, Transgenic
Ovalbumin genetics
Ovalbumin immunology
Peptides metabolism
Antigen Presentation
Antigens immunology
Dendritic Cells immunology
Peptides immunology
Protein Aggregates immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1550-6606
- Volume :
- 205
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 33067378
- Full Text :
- https://doi.org/10.4049/jimmunol.1901535