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Stimulation-induced differential redistributions of clathrin and clathrin-coated vesicles in axons compared to soma/dendrites.
- Source :
-
Molecular brain [Mol Brain] 2020 Oct 16; Vol. 13 (1), pp. 141. Date of Electronic Publication: 2020 Oct 16. - Publication Year :
- 2020
-
Abstract
- Clathrin-mediated endocytosis plays an important role in the recycling of synaptic vesicle in presynaptic terminals, and in the recycling of transmitter receptors in neuronal soma/dendrites. The present study uses electron microscopy (EM) and immunogold EM to document the different categories of clathrin-coated vesicles (CCV) and pits (CCP) in axons compared to soma/dendrites, and the depolarization-induced redistribution of clathrin in these two polarized compartments of the neuron. The size of CCVs in presynaptic terminals (~ 40 nm; similar to the size of synaptic vesicles) is considerably smaller than the size of CCVs in soma/dendrites (~ 90 nm). Furthermore, neuronal stimulation induces an increase in the number of CCV/CCP in presynaptic terminals, but a decrease in soma/dendrites. Immunogold labeling of clathrin revealed that in presynaptic terminals under resting conditions, the majority of clathrin molecules are unassembled and concentrated outside of synaptic vesicle clusters. Upon depolarization with high K <superscript>+</superscript> , label for clathrin became scattered among de-clustered synaptic vesicles and moved closer to the presynaptic active zone. In contrast to axons, clathrin-labeled CCVs and CCPs were prominent in soma/dendrites under resting conditions, and became inconspicuous upon depolarization with high K <superscript>+</superscript> . Thus, EM examination suggests that the regulation and mechanism of clathrin-mediated endocytosis differ between axon and dendrite, and that clathrin redistributes differently in these two neuronal compartments upon depolarization.
- Subjects :
- Animals
Axons ultrastructure
Cell Membrane metabolism
Cell Membrane ultrastructure
Clathrin-Coated Vesicles ultrastructure
Dendrites ultrastructure
Mice
Multivesicular Bodies metabolism
Multivesicular Bodies ultrastructure
Post-Synaptic Density metabolism
Presynaptic Terminals metabolism
Rats
Axons metabolism
Clathrin metabolism
Clathrin-Coated Vesicles metabolism
Dendrites metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1756-6606
- Volume :
- 13
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Molecular brain
- Publication Type :
- Academic Journal
- Accession number :
- 33066817
- Full Text :
- https://doi.org/10.1186/s13041-020-00683-5