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A post-insertion strategy for surface functionalization of bacterial and mammalian cell-derived extracellular vesicles.
- Source :
-
Biochimica et biophysica acta. General subjects [Biochim Biophys Acta Gen Subj] 2021 Apr; Vol. 1865 (4), pp. 129763. Date of Electronic Publication: 2020 Oct 14. - Publication Year :
- 2021
-
Abstract
- Extracellular vesicles (EVs) are nanoparticles which are released by cells from all three domains of life: Archaea, Bacteria and Eukarya. They can mediate cell-cell communication by transferring cargoes such as proteins and nucleic acids between cells. EVs receive great interest in both academia and industry as they have the potential to be natural drug carriers or vaccine candidates. However, limitations to their clinical translation exist as efficient isolation, loading, labelling and surface-engineering methods are lacking. In this article, we investigate a 'post-insertion' approach, which is commonly used in the functionalization of liposomes in the pharmaceutical field, on two different EV types: mammalian cell-derived EVs and bacteria-derived EVs. We aimed to find an easy and flexible approach to functionalize EVs, thereby improving the labelling, isolation, and surface-engineering.<br /> (Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Bacterial Outer Membrane ultrastructure
Blotting, Western methods
Cell Culture Techniques methods
Cell Line, Tumor
Electrophoresis, Polyacrylamide Gel methods
Extracellular Vesicles ultrastructure
Flow Cytometry methods
HEK293 Cells
Humans
Mice
Microscopy, Electron, Transmission methods
Surface Properties
Bacteria chemistry
Bacterial Outer Membrane chemistry
Extracellular Vesicles chemistry
Immunohistochemistry methods
Subjects
Details
- Language :
- English
- ISSN :
- 1872-8006
- Volume :
- 1865
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Biochimica et biophysica acta. General subjects
- Publication Type :
- Academic Journal
- Accession number :
- 33065252
- Full Text :
- https://doi.org/10.1016/j.bbagen.2020.129763