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Carnosic acid improves porcine early embryonic development by inhibiting the accumulation of reactive oxygen species.
- Source :
-
The Journal of reproduction and development [J Reprod Dev] 2020 Dec 22; Vol. 66 (6), pp. 555-562. Date of Electronic Publication: 2020 Oct 14. - Publication Year :
- 2020
-
Abstract
- Carnosic acid (CA), a natural catechol rosin diterpene, is used as an additive in animal feeds and human foods. However, the effects of CA on mammalian reproductive processes, especially early embryonic development, are unclear. In this study, we added CA to parthenogenetically activated porcine embryos in an in vitro culture medium to explore the influence of CA on apoptosis, proliferation, blastocyst formation, reactive oxygen species (ROS) levels, glutathione (GSH) levels, mitochondrial membrane potential, and embryonic development-related gene expression. The results showed that supplementation with 10 μM CA during in vitro culture significantly improved the cleavage rates, blastocyst formation rates, hatching rates, and total numbers of cells of parthenogenetically activated porcine embryos compared with no supplementation. More importantly, supplementation with CA also improved GSH levels and mitochondrial membrane potential, reduced natural ROS levels in blastomeres, upregulated Nanog, Sox2, Gata4, Cox2, Itga5, and Rictor expression, and downregulated Birc5 and Caspase3 expression. These results suggest that CA can improve early porcine embryonic development by regulating oxidative stress. This study elucidates the effects of CA on early embryonic development and their potential mechanisms, and provides new applications for improving the quality of in vitro-developed embryos.
- Subjects :
- Animals
Apoptosis
Blastocyst cytology
Cell Proliferation
Culture Media
Embryo Culture Techniques
Female
Gene Expression Profiling
Gene Expression Regulation
Glutathione metabolism
In Vitro Oocyte Maturation Techniques methods
Membrane Potential, Mitochondrial
Oxidative Stress
Parthenogenesis
Pregnancy
Pregnancy, Animal
Swine
Abietanes pharmacology
Embryonic Development drug effects
Reactive Oxygen Species
Subjects
Details
- Language :
- English
- ISSN :
- 1348-4400
- Volume :
- 66
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- The Journal of reproduction and development
- Publication Type :
- Academic Journal
- Accession number :
- 33055461
- Full Text :
- https://doi.org/10.1262/jrd.2020-086