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Nanotechnology enabled the enhancement of antitrypanosomal activity of piperine against Trypanosoma evansi.
- Source :
-
Experimental parasitology [Exp Parasitol] 2020 Dec; Vol. 219, pp. 108018. Date of Electronic Publication: 2020 Oct 10. - Publication Year :
- 2020
-
Abstract
- Nanoencapsulation is the promising approach to enhance the therapeutic potential of a drug. In the present investigation, piperine-loaded nanocapsules (NCs) was prepared and evaluated for antitrypanosomal activity against the parasite Trypanosoma evansi, a causative agent of trypanosomiasis. Piperine, a bioactive compound was selected as an alternative for drugs that have been used for the treatment of the disease from decades to overcome the toxic effects or drug resistance effect. Moreover, piperine has reported to possess therapeutic potential against other Trypanosoma spp. and has also been reported to cause reactive oxygen species (ROS) mediated effect in cancer cells that was the other reason for the selection. To date, piperine and its nanoformulations have not been evaluated for their growth inhibitory effect against T. evansi. Piperine-loaded NCs exhibited more significant antitrypanosomal effect at approximately three-times less IC <subscript>50</subscript> value 5.04 μM as compared to piperine (IC <subscript>50</subscript> -14.45 μM). Moreover, increased production of reactive oxygen species observed in the case of piperine-loaded NCs as that of pure piperine in the axenic culture of T. evansi. Furthermore, different concentrations of piperine-loaded NCs showed less cytotoxicity on horse peripheral blood mononuclear cells as liken to pure piperine. In conclusion, our results demonstrated that piperine-loaded NCs induced more generation of ROS that contributed inhibitory effect on the growth of Trypanosoma evansi as compared to pure drug.<br /> (Copyright © 2020 Elsevier Inc. All rights reserved.)
- Subjects :
- Alkaloids toxicity
Analysis of Variance
Animals
Benzodioxoles toxicity
Cytochrome P-450 Enzyme Inhibitors toxicity
Horses
Inhibitory Concentration 50
Leukocytes, Mononuclear drug effects
Nanocapsules
Piperidines toxicity
Polyunsaturated Alkamides toxicity
Reactive Oxygen Species analysis
Reactive Oxygen Species metabolism
Trypanosoma growth & development
Alkaloids pharmacology
Benzodioxoles pharmacology
Cytochrome P-450 Enzyme Inhibitors pharmacology
Piperidines pharmacology
Polyunsaturated Alkamides pharmacology
Trypanosoma drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2449
- Volume :
- 219
- Database :
- MEDLINE
- Journal :
- Experimental parasitology
- Publication Type :
- Academic Journal
- Accession number :
- 33049224
- Full Text :
- https://doi.org/10.1016/j.exppara.2020.108018