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Cytoskeleton systems contribute differently to the functional intrinsic properties of chondrospheres.
- Source :
-
Acta biomaterialia [Acta Biomater] 2020 Dec; Vol. 118, pp. 141-152. Date of Electronic Publication: 2020 Oct 10. - Publication Year :
- 2020
-
Abstract
- Cytoskeleton systems, actin microfilaments, microtubules (MTs) and intermediate filaments (IFs) provide the biomechanical stability and spatial organization in cells. To understand the specific contributions of each cytoskeleton systems to intrinsic properties of spheroids, we've scrutinized the effects of the cytoskeleton perturbants, cytochalasin D (Cyto D), nocodazole (Noc) and withaferin A (WFA) on fusion, spreading on adhesive surface, morphology and biomechanics of chondrospheres (CSs). We confirmed that treatment with Cyto D but not with Noc or WFA severely affected CSs fusion and spreading dynamics and significantly reduced biomechanical properties of cell aggregates. Noc treatment affected spheroids spreading but not the fusion and surprisingly enhanced their stiffness. Vimentin intermediate filaments (VIFs) reorganization affected CSs spreading only. The analysis of all three cytoskeleton systems contribution to spheroids intrinsic properties was performed for the first time.<br />Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2020. Published by Elsevier Ltd.)
- Subjects :
- Actin Cytoskeleton
Microtubules
Vimentin
Cytoskeleton
Intermediate Filaments
Subjects
Details
- Language :
- English
- ISSN :
- 1878-7568
- Volume :
- 118
- Database :
- MEDLINE
- Journal :
- Acta biomaterialia
- Publication Type :
- Academic Journal
- Accession number :
- 33045401
- Full Text :
- https://doi.org/10.1016/j.actbio.2020.10.007