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Structural component changes of erythrocytes caused by oxidative stress generated by indoxyl sulfate.
- Source :
-
Toxicology in vitro : an international journal published in association with BIBRA [Toxicol In Vitro] 2021 Feb; Vol. 70, pp. 105013. Date of Electronic Publication: 2020 Oct 08. - Publication Year :
- 2021
-
Abstract
- Indoxyl sulfate (IS) belongs to groups of uremic toxins binding to proteins. This compound may contribute to the generation of oxidative stress in chronic kidney disease (CKD) patients. We hypothesized that a high concentration of IS in the blood may induce structural changes of erythrocyte components and thus may contribute to CKD progression. In the present study, we evaluated the influence of IS on hemolysate and membrane proteins' conformational state, lipid membrane fluidity, and internal viscosity in erythrocytes. We examined thiols, carbonyl groups, peroxides, and TBARS levels in erythrocyte incubated with IS. The treatment of erythrocytes with IS led to increase in lipid membrane fluidity, decrease in the internal viscosity of the cells and the motion of the spin labels attached to hemolysate proteins. We did not observe conformational changes in plasma membrane proteins; however, in the plasma membranes of erythrocytes incubated with IS, a decrease in the content of thiol groups and increase in the carbonyls levels and peroxides and TBARS in comparison with the control was observed. The obtained results indicate that IS induces the oxidative damage of erythrocyte components. This may be an important factor that affects the functional properties of erythrocytes in CKD patients.<br /> (Copyright © 2020 Elsevier Ltd. All rights reserved.)
- Subjects :
- Cell Membrane drug effects
Cell Membrane metabolism
Erythrocytes metabolism
Erythrocytes physiology
Humans
Membrane Fluidity drug effects
Membrane Proteins metabolism
Oxidative Stress drug effects
Peroxides metabolism
Thiobarbituric Acid Reactive Substances metabolism
Erythrocytes drug effects
Indican toxicity
Subjects
Details
- Language :
- English
- ISSN :
- 1879-3177
- Volume :
- 70
- Database :
- MEDLINE
- Journal :
- Toxicology in vitro : an international journal published in association with BIBRA
- Publication Type :
- Academic Journal
- Accession number :
- 33038466
- Full Text :
- https://doi.org/10.1016/j.tiv.2020.105013