Back to Search
Start Over
Significant association of serum autoantibodies to TYMS, HAPLN1 and IGFBP5 with early stage canine malignant mammary tumours.
- Source :
-
Veterinary and comparative oncology [Vet Comp Oncol] 2021 Mar; Vol. 19 (1), pp. 172-182. Date of Electronic Publication: 2020 Oct 19. - Publication Year :
- 2021
-
Abstract
- Canine mammary tumours (CMTs) are the most prevalent neoplasms in female dogs. Despite the high incidence of such tumours, a lack of easily accessible biomarkers still impedes early diagnosis of malignant CMTs. Herein we identify thymidylate synthetase (TYMS), hyaluronan and proteoglycan link protein 1 (HAPLN1) and insulin-like growth factor-binding protein 5 (IGFBP5) as CMT antigens eliciting corresponding autoantibodies in CMT cases. We establish enzyme-linked immunosorbent assays (ELISAs) to detect autoantibodies to TYMS (TYMS-AAb), HAPLN1 (HAPLN1-AAb) and IGFBP5 (IGFBP5-AAb) in sera from 81 dogs with malignant CMTs (41 in Stage I), 24 with benign CMTs and 35 healthy controls. Levels of all the three autoantibodies are elevated in the malignant group compared with the healthy or the benign group; notably, the elevated autoantibody levels significantly correlate with the stage-I CMTs. For discriminating malignant CMTs from healthy control, the area under curve (AUC) of TYMS-AAb is 0.694 with specificity of 82.9% and sensitivity of 50.6%. The AUC of utilising HAPLN1-AAb for distinguishing the stage-I CMTs from healthy controls is 0.711 with specificity of 77.1% and sensitivity of 58.5%. In differentiating malignant CMTs from the benign, the AUC of IGFBP5-AAb reaches 0.696 with specificity of 70.8% and sensitivity of 67.9%, and a combination of IGFBP5-AAb and TYMS-AAb increases the AUC to 0.72. Finally, the AUC of combined HAPLN1-AAb and IGFBP5-AAb in discriminating the stage-I CMTs from the benign achieves 0.731. Collectively, this study highlights a significant association of the three serum autoantibodies with early stage malignant CMTs.<br /> (© 2020 John Wiley & Sons Ltd.)
- Subjects :
- Animals
Autoantibodies blood
Biomarkers, Tumor
Carcinoma metabolism
Carcinoma veterinary
Dog Diseases blood
Dogs
Extracellular Matrix Proteins genetics
Female
Gene Expression Regulation, Neoplastic
Insulin-Like Growth Factor Binding Protein 5 genetics
Mammary Neoplasms, Animal metabolism
Neoplasm Staging veterinary
Proteoglycans genetics
Thymidylate Synthase genetics
Dog Diseases immunology
Extracellular Matrix Proteins metabolism
Insulin-Like Growth Factor Binding Protein 5 metabolism
Mammary Neoplasms, Animal immunology
Proteoglycans metabolism
Thymidylate Synthase metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1476-5829
- Volume :
- 19
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Veterinary and comparative oncology
- Publication Type :
- Academic Journal
- Accession number :
- 33038064
- Full Text :
- https://doi.org/10.1111/vco.12657