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One-pot synthesis of hyaluronic acid-coated gold nanoparticles as SERS substrate for the determination of hyaluronidase activity.
- Source :
-
Mikrochimica acta [Mikrochim Acta] 2020 Oct 10; Vol. 187 (11), pp. 604. Date of Electronic Publication: 2020 Oct 10. - Publication Year :
- 2020
-
Abstract
- A novel ultrasensitive surface-enhanced Raman spectroscopy (SERS)-based method was developed for the determination of hyaluronidase (HAase), which was based on hyaluronic acid-coated gold nanoparticles (HA-AuNPs) as a substrate, via a facile one-pot method. The detection mechanism is based on HAase which can hydrolyze HA on HA-AuNPs into hyaluronic acid oligomers, causing the originally uniformly dispersed HA-AuNPs to be disintegrated into many smaller HA-AuNPs. These oligomers in turn increase the surface shielding of AuNPs, resulting in high aggregation tendencies. As a result, the original SERS substrate was disassembled, leading to a weakening of the SERS signal at 1173 cm <superscript>-1</superscript> . Malachite green was also used as a Raman probe to detect the change of SERS peak intensity and to quantify HAase. Compared with other methods for the determination of HAase, this method is more convenient and efficient; its determination limit was 0.4 mU mL <superscript>-1</superscript> . The recoveries of HAase spiked into human urine samples ranged from 97.2 to 103.9%.
Details
- Language :
- English
- ISSN :
- 1436-5073
- Volume :
- 187
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Mikrochimica acta
- Publication Type :
- Academic Journal
- Accession number :
- 33037925
- Full Text :
- https://doi.org/10.1007/s00604-020-04566-3