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Salt effects on bacteriophage T7-I.
- Source :
-
Physiological chemistry and physics and medical NMR [Physiol Chem Phys Med NMR] 1987; Vol. 19 (1), pp. 59-66. - Publication Year :
- 1987
-
Abstract
- The thermal denaturation as a measure of the structural stability of the nucleoprotein in bacteriophage T7 has been studied in dependence of the ionic environment. Optical density and circular dichroism melting curves measured at wavelengths characterizing either the DNA or protein conformational changes were compared to identify different steps of the denaturation and to follow the effect of the ions. Monovalent salts strengthen the helical structure of intraphage DNA logarithmically in the way as they do in the case of isolated double-stranded DNA. Mg2+ and Ca2+ at very low concentrations stabilize the DNA helicity. Higher divalent ion concentrations decrease the stability of the double helix because of the repulsive ionic interactions. The high structural sensitivity of DNA in the presence of Mg2+ and Ca2+ in this "in situ" environment can be related to the biological role of these ions.
- Subjects :
- Calcium pharmacology
DNA, Viral metabolism
Deoxyribonucleoproteins metabolism
Escherichia coli physiology
Magnesium pharmacology
Nucleic Acid Conformation
Nucleic Acid Denaturation
Osmolar Concentration
T-Phages physiology
DNA, Viral drug effects
Escherichia coli drug effects
T-Phages drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 0748-6642
- Volume :
- 19
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Physiological chemistry and physics and medical NMR
- Publication Type :
- Academic Journal
- Accession number :
- 3303075