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Effect of Curcuma zedoaria hydro-alcoholic extract on learning, memory deficits and oxidative damage of brain tissue following seizures induced by pentylenetetrazole in rat.
- Source :
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Behavioral and brain functions : BBF [Behav Brain Funct] 2020 Oct 06; Vol. 16 (1), pp. 7. Date of Electronic Publication: 2020 Oct 06. - Publication Year :
- 2020
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Abstract
- Background: Previous studies have shown that seizures can cause cognitive disorders. On the other hand, the Curcuma zedoaria (CZ) has beneficial effects on the nervous system. However, there is little information on the possible effects of the CZ extract on seizures. The aim of this study was to investigate the possible effects of CZ extract on cognitive impairment and oxidative stress induced by epilepsy in rats.<br />Methods: Rats were randomly divided into different groups. In all rats (except the sham group), kindling was performed by intraperitoneal injection of pentylenetetrazol (PTZ) at a dose of 35 mg/kg every 48 h for 14 days. Positive group received 2 mg/kg diazepam + PTZ; treatment groups received 100, 200 or 400 mg/kg CZ extract + PTZ; and one group received 0.5 mg/kg flumazenil and CZ extract + PTZ. Shuttle box and Morris Water Maze tests were used to measure memory and learning. On the last day of treatments PTZ injection was at dose of 60 mg/kg, tonic seizure threshold and mortality rate were recorded in each group. After deep anesthesia, blood was drawn from the rats' hearts and the hippocampus of all rats was removed.<br />Results: Statistical analysis of the data showed that the CZ extract significantly increased the tonic seizure threshold and reduced the pentylenetetrazol-induced mortality and the extract dose of 400 mg/kg was selected as the most effective dose compared to the other doses. It was also found that flumazenil (a GABA <subscript>A</subscript> receptor antagonist) reduced the tonic seizure threshold compared to the effective dose of the extract. The results of shuttle box and Morris water maze behavioral tests showed that memory and learning decreased in the negative control group and the CZ extract treatment improved memory and learning in rats. The CZ extract also increased antioxidant capacity, decreased MDA and NO in the brain and serum of pre-treated groups in compared to the negative control group.<br />Conclusion: It is concluded that the CZ extract has beneficial effects on learning and memory impairment in PTZ-induced epilepsy model, which has been associated with antioxidant effects in the brain or possibly exerts its effects through the GABAergic system.
- Subjects :
- Animals
Anticonvulsants therapeutic use
Antioxidants pharmacology
Convulsants
Flumazenil therapeutic use
GABA Modulators therapeutic use
Learning Disabilities psychology
Male
Malondialdehyde metabolism
Maze Learning
Memory Disorders psychology
Nitric Oxide metabolism
Pentylenetetrazole
Rats
Rats, Wistar
Seizures chemically induced
Brain Chemistry drug effects
Curcuma chemistry
Learning Disabilities drug therapy
Memory Disorders drug therapy
Oxidative Stress drug effects
Plant Extracts therapeutic use
Seizures psychology
Subjects
Details
- Language :
- English
- ISSN :
- 1744-9081
- Volume :
- 16
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Behavioral and brain functions : BBF
- Publication Type :
- Academic Journal
- Accession number :
- 33023622
- Full Text :
- https://doi.org/10.1186/s12993-020-00169-3