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A Dual-Antigen Enzyme-Linked Immunosorbent Assay Allows the Assessment of Severe Acute Respiratory Syndrome Coronavirus 2 Antibody Seroprevalence in a Low-Transmission Setting.
- Source :
-
The Journal of infectious diseases [J Infect Dis] 2021 Jan 04; Vol. 223 (1), pp. 10-14. - Publication Year :
- 2021
-
Abstract
- Estimates of seroprevalence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies have been hampered by inadequate assay sensitivity and specificity. Using an enzyme-linked immunosorbent assay-based approach that combines data about immunoglobulin G responses to both the nucleocapsid and spike receptor binding domain antigens, we show that excellent sensitivity and specificity can be achieved. We used this assay to assess the frequency of virus-specific antibodies in a cohort of elective surgery patients in Australia and estimated seroprevalence in Australia to be 0.28% (95% Confidence Interval, 0-1.15%). These data confirm the low level of transmission of SARS-CoV-2 in Australia before July 2020 and validate the specificity of our assay.<br /> (© The Author(s) 2020. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: journals.permissions@oup.com.)
- Subjects :
- Antigens, Viral immunology
Australia
COVID-19 immunology
Coronavirus Nucleocapsid Proteins immunology
Humans
Immunoglobulin G analysis
Phosphoproteins immunology
Sensitivity and Specificity
Spike Glycoprotein, Coronavirus immunology
Antibodies, Viral analysis
COVID-19 diagnosis
Enzyme-Linked Immunosorbent Assay
Seroepidemiologic Studies
Subjects
Details
- Language :
- English
- ISSN :
- 1537-6613
- Volume :
- 223
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- The Journal of infectious diseases
- Publication Type :
- Academic Journal
- Accession number :
- 33009908
- Full Text :
- https://doi.org/10.1093/infdis/jiaa623