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Mechanism of self/nonself-discrimination in Brassica self-incompatibility.
- Source :
-
Nature communications [Nat Commun] 2020 Oct 01; Vol. 11 (1), pp. 4916. Date of Electronic Publication: 2020 Oct 01. - Publication Year :
- 2020
-
Abstract
- Self-incompatibility (SI) is a breeding system that promotes cross-fertilization. In Brassica, pollen rejection is induced by a haplotype-specific interaction between pistil determinant SRK (S receptor kinase) and pollen determinant SP11 (S-locus Protein 11, also named SCR) from the S-locus. Although the structure of the B. rapa S <subscript>9</subscript> -SRK ectodomain (eSRK) and S <subscript>9</subscript> -SP11 complex has been determined, it remains unclear how SRK discriminates self- and nonself-SP11. Here, we uncover the detailed mechanism of self/nonself-discrimination in Brassica SI by determining the S <subscript>8</subscript> -eSRK-S <subscript>8</subscript> -SP11 crystal structure and performing molecular dynamics (MD) simulations. Comprehensive binding analysis of eSRK and SP11 structures reveals that the binding free energies are most stable for cognate eSRK-SP11 combinations. Residue-based contribution analysis suggests that the modes of eSRK-SP11 interactions differ between intra- and inter-subgroup (a group of phylogenetically neighboring haplotypes) combinations. Our data establish a model of self/nonself-discrimination in Brassica SI.
- Subjects :
- Animals
Crystallography
Flowers metabolism
Haplotypes
Molecular Dynamics Simulation
Plant Proteins genetics
Plant Proteins ultrastructure
Pollen metabolism
Protein Binding physiology
Protein Domains physiology
Protein Kinases genetics
Protein Kinases ultrastructure
Recombinant Proteins genetics
Recombinant Proteins metabolism
Recombinant Proteins ultrastructure
Sf9 Cells
Spodoptera
Brassica rapa physiology
Plant Breeding
Plant Proteins metabolism
Protein Kinases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2041-1723
- Volume :
- 11
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Nature communications
- Publication Type :
- Academic Journal
- Accession number :
- 33004803
- Full Text :
- https://doi.org/10.1038/s41467-020-18698-w