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Effects of myofiber isolation technique on sarcolemma biomechanics.

Authors :
Garcia-Pelagio KP
Pratt SJP
Lovering RM
Source :
BioTechniques [Biotechniques] 2020 Nov; Vol. 69 (5), pp. 388-391. Date of Electronic Publication: 2020 Oct 01.
Publication Year :
2020

Abstract

Isolated myofibers are commonly used to understand the function of skeletal muscle in vivo . This can involve single isolated myofibers obtained from dissection or from enzymatic dissociation. Isolation via dissection allows control of sarcomere length and preserves tendon attachment but is labor-intensive, time-consuming and yields few viable myofibers. In contrast, enzymatic dissociation is fast and facile, produces hundreds of myofibers, and more importantly reduces the number of muscles/animals needed for studies. Biomechanical properties of the sarcolemma have been studied using myofibers from the extensor digitorum longus, but this has been limited to dissected myofibers, making data collection slow and difficult. We have modified this tool to perform biomechanical measurements of the sarcolemma in dissociated myofibers from the flexor digitorum brevis.

Details

Language :
English
ISSN :
1940-9818
Volume :
69
Issue :
5
Database :
MEDLINE
Journal :
BioTechniques
Publication Type :
Academic Journal
Accession number :
33000629
Full Text :
https://doi.org/10.2144/btn-2020-0087