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CircRNA Circ_0001721 Promotes the Progression of Osteosarcoma Through miR-372-3p/MAPK7 Axis.
- Source :
-
Cancer management and research [Cancer Manag Res] 2020 Sep 11; Vol. 12, pp. 8287-8302. Date of Electronic Publication: 2020 Sep 11 (Print Publication: 2020). - Publication Year :
- 2020
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Abstract
- Background: Osteosarcoma (OS) is the most common bone tumor. Many studies have reported that circular RNAs (circRNAs) play an important role in the development of a variety of human cancers. However, the underlying mechanism of circ&#95;0001721 in regulating osteosarcoma progression remains unknown.<br />Materials and Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the levels of circ&#95;0001721, miR-372-3p, and mitogen-activated protein kinase 7 (MAPK7) in osteosarcoma tissues and cells. Besides, glycolysis was investigated by glucose consumption, lactate production and hexokinase II (HK2) protein level. Cell proliferation and apoptosis were determined by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) and flow cytometry, separately. Cell migration and invasion were determined by transwell assay. Moreover, the protein levels of HK2 and epithelial-to-mesenchymal transition (EMT) markers were determined by Western blot analysis. The relationship between miR-372-3p and circ&#95;0001721 or MAPK7 was predicated by starbase v3.0 and confirmed by dual-luciferase reporter assay or RNA binding protein immunoprecipitation (RIP) assay. Murine xenograft model was established to investigate the role of circ&#95;0001721 in vivo.<br />Results: The levels of circ&#95;0001721 and MAPK7 were upregulated in osteosarcoma tissues and cells, while miR-372-3p was downregulated. Knockdown of circ&#95;0001721 inhibited glycolysis, cell proliferation, cell migration, invasion and epithelial-to-mesenchymal transition (EMT), and promoted apoptosis. Circ&#95;0001721 was validated as a sponge of miR-372-3p and mediated glycolysis, cell proliferation, apoptosis, migration, invasion, and EMT of osteosarcoma cells through miR-372-3p. MAPK7 was a target of miR-372-3p and overexpression of MAPK7 attenuated anti-cancer role of miR-372-3p in OS cells. Further studies revealed that circ&#95;0001721 regulates MAPK7 expression via sponging miR-372-3-p. Finally, knockdown of circ&#95;0001721 inhibited tumor progression in vivo.<br />Conclusion: Circ&#95;0001721 promoted osteosarcoma development through the miR-372-3p/MAPK7 axis.<br />Competing Interests: The authors report no conflicts of interest in this work.<br /> (© 2020 Gao et al.)
Details
- Language :
- English
- ISSN :
- 1179-1322
- Volume :
- 12
- Database :
- MEDLINE
- Journal :
- Cancer management and research
- Publication Type :
- Academic Journal
- Accession number :
- 32982424
- Full Text :
- https://doi.org/10.2147/CMAR.S244527