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DAPI-pUC8 complex: a tool to investigate biological effects of nucleic acid-drug interaction.

DAPI-pUC8 complex: a tool to investigate biological effects of nucleic acid-drug interaction.

Authors :
Palu G
Valisena S
Barcellona ML
Masotti L
Meloni GA
Source :
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 1987 May 29; Vol. 145 (1), pp. 40-5.
Publication Year :
1987

Abstract

A complex consisting of pUC8, a 1.8 Md plasmid, and 4'-6-diamidino-2-phenylindole, a DNA-binding agent, has been performed in vitro under different conditions of ionic strength, and used to transform competent cells. A strong interference with the plasmid-coded activities, related to the P/D ratio where at the DNA-drug complex was formed, was shown to occur. Since the compound does not inhibit the uptake process neither affects plasmid activity once dissociated at high ionic strength, it is likely to be acting from inside the cell while still in the form of a DNA-adduct. This system is proposed as a useful tool to investigate the effects on target genes of drugs endowed with DNA sequence specificity.

Details

Language :
English
ISSN :
0006-291X
Volume :
145
Issue :
1
Database :
MEDLINE
Journal :
Biochemical and biophysical research communications
Publication Type :
Academic Journal
Accession number :
3297056
Full Text :
https://doi.org/10.1016/0006-291x(87)91284-8