Back to Search
Start Over
Delta or Omega? Δ12 (ω6) fatty acid desaturases count 3C after the pre-existing double bond.
- Source :
-
Biochimie [Biochimie] 2020 Dec; Vol. 179, pp. 46-53. Date of Electronic Publication: 2020 Sep 16. - Publication Year :
- 2020
-
Abstract
- Fatty acid desaturases (FADs) represent a class of oxygen-dependent enzymes that dehydrogenate C-C bonds in the fatty acids (FAs) producing unsaturated CC double bonds that markedly change the properties of biological membranes. FADs are highly specific towards their acyl substrates, the position and configuration of the introduced double bonds. The double bond positioning of soluble acyl-carrier-protein Δ9-FADs was determined relative to the carboxyl end of a FA. Similar mode was suggested for the acyl-lipid Δ12-FADs (also known as ω6-FADs), however, their exact counting order remain unknown. Here we used monounsaturated odd- (17:1Δ <superscript>10</superscript> ) and even-chain (18:1Δ <superscript>11</superscript> ) FAs to show that acyl-lipid Δ12-FADs of, at least, two cyanobacterial species, Gloeobacter violaceus and Synechocystis sp. strain PCC 6803, use neither end of the fatty acid (Δ or ω) as a counting reference point; but count three carbons toward the methyl end from an existing double bond in the monoene precursors irrespective of a FA chain length.<br />Competing Interests: Declaration of competing interest Authors declare no conflict of interests.<br /> (Copyright © 2020 Elsevier B.V. and Société Française de Biochimie et Biologie Moléculaire (SFBBM). All rights reserved.)
- Subjects :
- Carbon metabolism
Cyanobacteria chemistry
Cyanobacteria enzymology
Fatty Acid Desaturases genetics
Fatty Acid Desaturases metabolism
Fatty Acids, Monounsaturated isolation & purification
Fatty Acids, Monounsaturated metabolism
Galactolipids analysis
Glycolipids analysis
Lipid Metabolism
Phosphatidylglycerols analysis
Spectrometry, Mass, Electrospray Ionization
Synechococcus chemistry
Synechococcus enzymology
Synechocystis chemistry
Synechocystis enzymology
Carbon chemistry
Fatty Acid Desaturases chemistry
Fatty Acid Desaturases supply & distribution
Fatty Acids, Monounsaturated chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1638-6183
- Volume :
- 179
- Database :
- MEDLINE
- Journal :
- Biochimie
- Publication Type :
- Academic Journal
- Accession number :
- 32946991
- Full Text :
- https://doi.org/10.1016/j.biochi.2020.09.009