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C-type lectin 5, a novel pattern recognition receptor for the JAK/STAT signaling pathway in Bombyx mori.
- Source :
-
Journal of invertebrate pathology [J Invertebr Pathol] 2021 Feb; Vol. 179, pp. 107473. Date of Electronic Publication: 2020 Sep 16. - Publication Year :
- 2021
-
Abstract
- The Janus kinase/signal transducer and activator of transcription cascade transduction (JAK/STAT) signaling pathway is highly conserved in mammals, but the pattern recognition receptors (PRRs) and their functions are unclear. We found that the expression pattern of Bombyx mori C-type lectin 5 (BmCTL 5) had a synergy relevance with the JAK/STAT signaling pathway against Beauveria bassiana. An RNAi assay, subcellular localization analysis, yeast two-hybrid technique, protein recruitment experiment and pathogen infection tests were used to explore the roles of BmCTL 5 in the JAK/STAT signaling pathway. Knock-down of the BmCTL 5 suppressed the JAK/STAT signaling pathway and the PO cascade of nodule melanization. BmCTL 5 is located in the cytomembrane and interacted with BmHOP both in yeast and B. mori ovary cells N (BmN cells). BmCTL 5 and the JAK/STAT signaling pathway was activated by B. bassiana but only slightly activated by B. mori cytoplasmic polyhedrosis virus (BmCPV), Nosema bombycis and bacteria LPS. These findings suggest that BmCTL 5 might be an important PRR for the JAK/STAT signaling pathway and may mediate the nodule melanization for fungi infection. These data provide insights into the immune mechanism of the JAK/STAT signaling pathway in insects and aid understanding of the mechanism of the JAK/STAT signaling pathway and adaptive immune systems in mammals.<br /> (Copyright © 2020. Published by Elsevier Inc.)
- Subjects :
- Animals
Bombyx growth & development
Bombyx metabolism
Insect Proteins metabolism
Larva genetics
Larva growth & development
Larva metabolism
Larva microbiology
Lectins, C-Type metabolism
Receptors, Pattern Recognition metabolism
Beauveria physiology
Bombyx genetics
Bombyx microbiology
Insect Proteins genetics
Lectins, C-Type genetics
Receptors, Pattern Recognition genetics
Signal Transduction
Subjects
Details
- Language :
- English
- ISSN :
- 1096-0805
- Volume :
- 179
- Database :
- MEDLINE
- Journal :
- Journal of invertebrate pathology
- Publication Type :
- Academic Journal
- Accession number :
- 32946913
- Full Text :
- https://doi.org/10.1016/j.jip.2020.107473