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A Granulocytic Signature Identifies COVID-19 and Its Severity.
- Source :
-
The Journal of infectious diseases [J Infect Dis] 2020 Nov 13; Vol. 222 (12), pp. 1985-1996. - Publication Year :
- 2020
-
Abstract
- Background: An unbiased approach to SARS-CoV-2-induced immune dysregulation has not been undertaken so far. We aimed to identify previously unreported immune markers able to discriminate COVID-19 patients from healthy controls and to predict mild and severe disease.<br />Methods: An observational, prospective, multicentric study was conducted in patients with confirmed mild/moderate (n = 7) and severe (n = 19) COVID-19. Immunophenotyping of whole-blood leukocytes was performed in patients upon hospital ward or intensive care unit admission and in healthy controls (n = 25). Clinically relevant associations were identified through unsupervised analysis.<br />Results: Granulocytic (neutrophil, eosinophil, and basophil) markers were enriched during COVID-19 and discriminated between patients with mild and severe disease. Increased counts of CD15+CD16+ neutrophils, decreased granulocytic expression of integrin CD11b, and Th2-related CRTH2 downregulation in eosinophils and basophils established a COVID-19 signature. Severity was associated with emergence of PD-L1 checkpoint expression in basophils and eosinophils. This granulocytic signature was accompanied by monocyte and lymphocyte immunoparalysis. Correlation with validated clinical scores supported pathophysiological relevance.<br />Conclusions: Phenotypic markers of circulating granulocytes are strong discriminators between infected and uninfected individuals as well as between severity stages. COVID-19 alters the frequency and functional phenotypes of granulocyte subsets with emergence of CRTH2 as a disease biomarker.<br /> (© The Author(s) 2020. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: journals.permissions@oup.com.)
- Subjects :
- Adult
Aged
Biomarkers metabolism
CD11b Antigen immunology
COVID-19 blood
COVID-19 diagnosis
Female
France
Humans
Immunophenotyping
Leukocyte Count
Lymphocytes immunology
Male
Middle Aged
Monocytes immunology
Prospective Studies
SARS-CoV-2
Severity of Illness Index
COVID-19 immunology
Granulocytes immunology
Receptors, Immunologic metabolism
Receptors, Prostaglandin metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1537-6613
- Volume :
- 222
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- The Journal of infectious diseases
- Publication Type :
- Academic Journal
- Accession number :
- 32941618
- Full Text :
- https://doi.org/10.1093/infdis/jiaa591