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Analysis of PEG 400 in perfusate samples by aqueous normal phase (ANP) chromatography with evaporative light scattering detection.

Authors :
Webster GK
Elliott A
Dahan A
Miller JM
Source :
Analytical methods : advancing methods and applications [Anal Methods] 2011 Mar 01; Vol. 3 (3), pp. 742-744.
Publication Year :
2011

Abstract

A rapid method for the analysis of polyethylene glycol (PEG 400) in perfusate samples is presented. Because PEG 400 lacks a strong chromophore in the UV range, detection was accomplished using evaporative light scattering detection (ELSD). In order to optimize the ELSD signal performance for a volatile mobile phase, the chromatographic separation was optimized using aqueous normal phase conditions on a Cogent® Diamond Hydride column.

Details

Language :
English
ISSN :
1759-9679
Volume :
3
Issue :
3
Database :
MEDLINE
Journal :
Analytical methods : advancing methods and applications
Publication Type :
Academic Journal
Accession number :
32938100
Full Text :
https://doi.org/10.1039/c0ay00698j