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Roseomonas harenae sp. nov., from desert gravel soil.
- Source :
-
International journal of systematic and evolutionary microbiology [Int J Syst Evol Microbiol] 2020 Nov; Vol. 70 (11), pp. 5711-5716. - Publication Year :
- 2020
-
Abstract
- A Gram-stain-negative, aerobic, non-motile, pink-pigmented, coccus bacterium, designated CPCC 101081 <superscript>T</superscript> , was isolated from a gravel soil sample collected from Badain Jara desert, PR China. Growth of the isolate occurred at 10-37 °C and pH 5.0-8.0, with optimal growth at 28-32 °C and pH 7.0, respectively. The major cellular fatty acids were summed feature 8 (C <subscript>18:1</subscript> ω7 c /C <subscript>18:1</subscript> ω6 c ), summed feature 3 (C <subscript>16:1</subscript> ω6 c /C <subscript>16:1</subscript> ω7 c ) and C <subscript>18:1</subscript> 2-OH. Q-10 was detected as the main respiratory quinone. Diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, an unidentified phospholipid, an amino-containing lipid and an unidentified glycophospholipid were examined in the polar lipids extraction. The 16S rRNA gene sequence comparison of strain CPCC 101081 <superscript>T</superscript> with the available sequences in the GenBank database showed that the isolate was closely related to members of the genus Rosenomonas , with the highest similarity to Roseomonas rosea DSM 14916 <superscript>T</superscript> (97.4 %). In the phylogenetic trees based on 16S rRNA gene sequences and the core genomes, strain CPCC 101081 <superscript>T</superscript> was included within the clade of the genus Roseomonas , representing a species level, with the closest neighbor of R. rosea DSM 14916 <superscript>T</superscript> . The genomic DNA G+C content was 68.7 mol%. The average nucleotide identity and the digital DNA-DNA hybridization values between strain CPCC 101081 <superscript>T</superscript> and the related type strains of the genus Roseomonas were all far lower than the cut-off values for definition species. On the basis of above phenotypic and genotypic characteristics, strain CPCC 101081 <superscript>T</superscript> is proposed to represent a novel species of the genus Roseomonas with the name Roseomonas harenae sp. nov. strain CPCC 101081 <superscript>T</superscript> (=KCTC 62852 <superscript>T</superscript> =NBRC 113512 <superscript>T</superscript> ) is the type strain of the species.
- Subjects :
- Bacterial Typing Techniques
Base Composition
China
DNA, Bacterial genetics
Fatty Acids chemistry
Methylobacteriaceae isolation & purification
Nucleic Acid Hybridization
Phospholipids chemistry
RNA, Ribosomal, 16S genetics
Sequence Analysis, DNA
Ubiquinone analogs & derivatives
Ubiquinone chemistry
Desert Climate
Methylobacteriaceae classification
Phylogeny
Soil Microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1466-5034
- Volume :
- 70
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- International journal of systematic and evolutionary microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 32936752
- Full Text :
- https://doi.org/10.1099/ijsem.0.004467