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FSH1 encodes lysophospholipase activity in Saccharomyces cerevisiae.
- Source :
-
Biotechnology letters [Biotechnol Lett] 2021 Jan; Vol. 43 (1), pp. 279-286. Date of Electronic Publication: 2020 Sep 12. - Publication Year :
- 2021
-
Abstract
- Objectives: To elucidate the role of FSH1 (family of serine hydrolase) in lipid homeostasis.<br />Results: Proteins in various species containing alpha/beta hydrolase domain are known to be involved in lipid metabolism. In silico analysis of the FSH1 gene in Saccharomyces cerevisiae revealed the presence of alpha/beta hydrolase domain (ABHD) and a lipase motif (GXSXG), however its function in lipid metabolism remained elusive. The overexpression of FSH1 in WT and fsh1Δ cells showed a significant reduction in the cellular phospholipid levels and an increase in the triacylglycerol levels and lipid droplet (LD) number. Furthermore, the purified recombinant protein Fsh1p was identified as a lysophospholipase that specifically acts on lysophosphatidylserine (LPS) and impacts the lipid homeostasis in S. cerevisiae.<br />Conclusions: These results depicted that Fsh1p has a role on lipid homeostasis and is a lysophospholipase that hydrolyzes lysophosphatidylserine (LPS).
- Subjects :
- Lipid Droplets metabolism
Lipid Metabolism genetics
Lysophospholipids metabolism
Phospholipids metabolism
Lysophospholipase genetics
Lysophospholipase metabolism
Saccharomyces cerevisiae enzymology
Saccharomyces cerevisiae metabolism
Saccharomyces cerevisiae Proteins genetics
Saccharomyces cerevisiae Proteins metabolism
Serine Proteases genetics
Serine Proteases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1573-6776
- Volume :
- 43
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Biotechnology letters
- Publication Type :
- Academic Journal
- Accession number :
- 32920715
- Full Text :
- https://doi.org/10.1007/s10529-020-03004-x