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NRAS Q61K melanoma tumor formation is reduced by p38-MAPK14 activation in zebrafish models and NRAS-mutated human melanoma cells.
- Source :
-
Pigment cell & melanoma research [Pigment Cell Melanoma Res] 2021 Mar; Vol. 34 (2), pp. 150-162. Date of Electronic Publication: 2020 Sep 24. - Publication Year :
- 2021
-
Abstract
- Oncogenic BRAF and NRAS mutations drive human melanoma initiation. We used transgenic zebrafish to model NRAS-mutant melanoma, and the rapid tumor onset allowed us to study candidate tumor suppressors. We identified P38α-MAPK14 as a potential tumor suppressor in The Cancer Genome Atlas melanoma cohort of NRAS-mutant melanomas, and overexpression significantly increased the time to tumor onset in transgenic zebrafish with NRAS-driven melanoma. Pharmacological activation of P38α-MAPK14 using anisomycin reduced in vitro viability of melanoma cultures, which we confirmed by stable overexpression of p38α. We observed that the viability of MEK inhibitor resistant melanoma cells could be reduced by combined treatment of anisomycin and MEK inhibition. Our study demonstrates that activating the p38α-MAPK14 pathway in the presence of oncogenic NRAS abrogates melanoma in vitro and in vivo. SIGNIFICANCE: The significance of our study is in the accountability of NRAS mutations in melanoma. We demonstrate here that activation of p38α-MAPK14 pathway can abrogate NRAS-mutant melanoma which is contrary to the previously published role of p38α-MAPK14 pathway in BRAF mutant melanoma. These results implicate that BRAF and NRAS-mutant melanoma may not be identical biologically. We also demonstrate the translational benefit of our study by using a small molecule compound-anisomycin (already in use for other diseases in clinical trials) to activate p38α-MAPK14 pathway.<br /> (© 2020 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
- Subjects :
- Animals
Anisomycin pharmacology
Apoptosis
Cell Proliferation
Humans
Melanoma genetics
Melanoma metabolism
Melanoma pathology
Mitogen-Activated Protein Kinase 14 antagonists & inhibitors
Mitogen-Activated Protein Kinase 14 genetics
Protein Kinase Inhibitors pharmacology
Protein Synthesis Inhibitors pharmacology
Tumor Cells, Cultured
Zebrafish
GTP Phosphohydrolases genetics
Gene Expression Regulation, Neoplastic drug effects
Melanoma prevention & control
Membrane Proteins genetics
Mitogen-Activated Protein Kinase 14 metabolism
Mutation
Subjects
Details
- Language :
- English
- ISSN :
- 1755-148X
- Volume :
- 34
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Pigment cell & melanoma research
- Publication Type :
- Academic Journal
- Accession number :
- 32910840
- Full Text :
- https://doi.org/10.1111/pcmr.12925