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Chicken Ovotransferrin Variants OTF B and OTF C Harboring Substitution of GAT (Asp) to AAT (Asn) in the Codon 500 and their Antimicrobial Activity.
- Source :
-
The journal of poultry science [J Poult Sci] 2016 Oct 25; Vol. 53 (4), pp. 257-263. - Publication Year :
- 2016
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Abstract
- Chicken egg white ovotransferrin (OTF) has been reported to exist in three electrophoretic variants (OTF <superscript>A</superscript> , OTF <superscript>B</superscript> and OTF <superscript>C</superscript> ). In this report, we identified a causal polymorphism between the OTF <superscript>B</superscript> and OTF <superscript>C</superscript> variants in Japanese and Taiwanese native chickens and compared the antibacterial activity between these two variants. The cDNA sequence analyses from Satsumadori oviducts revealed that three non-synonymous SNPs T1809G (Ser52Ala), A2258G (Ile96Val) and G7823A (Asp500Asn) corresponded to the OTF electrophoretic phenotypes. Of the three SNPs, the G7823A mutation perfectly corresponded to the electrophoretic phenotypes OTF <superscript>B</superscript> (G/G, Asp500Asp), OTF <superscript>B/C</superscript> (G/A, Asp500Asn) and OTF <superscript>C</superscript> (A/A, Asn500Asn) in three chicken populations. The variants OTF <superscript>B</superscript> and OTF <superscript>C</superscript> exhibited similar antibacterial potency against Gram-positive and Gram-negative bacteria. This study provides, for the first time, molecular information on polymorphism of OTF <superscript>B</superscript> and OTF <superscript>C</superscript> variants of chicken ovotransferrin and its effect on the antimicrobial activity of the respective variants.<br /> (2016, Japan Poultry Science Association.)
Details
- Language :
- English
- ISSN :
- 1349-0486
- Volume :
- 53
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- The journal of poultry science
- Publication Type :
- Academic Journal
- Accession number :
- 32908392
- Full Text :
- https://doi.org/10.2141/jpsa.0160055