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Biocatalytic production of Ɛ-caprolactone using Geotrichum candidum cells immobilized on functionalized silica.
- Source :
-
Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2020 Oct; Vol. 104 (20), pp. 8887-8895. Date of Electronic Publication: 2020 Sep 09. - Publication Year :
- 2020
-
Abstract
- Immobilization of the Geotrichum candidum (CCT 1205) cell with functionalized silica creates promising biocatalysts for production of ɛ-caprolactone. The results obtained by immobilization of the whole cell on SiO <subscript>2</subscript> -NH <subscript>2</subscript> and SiO <subscript>2</subscript> -SH supports indicate that the presence of reactive functional groups on the support may promote effective chemical bonds with the cell walls resulting the decreased dehydrogenases enzyme activity (5% yield in less than 2h) and consequently, increased Baeyer-Villiger monooxygenases enzyme activity with redacting of 25% of time reaction when is used SiO <subscript>2</subscript> -NH <subscript>2</subscript> as support and 50% through use of SiO <subscript>2</subscript> -SH as support relative to free cells when cyclohexanone is used as a substrate. The catalysts SiO <subscript>2</subscript> -NH <subscript>2</subscript> -Geotrichum candidum and SiO <subscript>2</subscript> -SH-Geotrichum candidum were recycling and reused in the ɛ-caprolactone synthesis from cyclohexanone, and the biocatalysts promoted a quantitative conversion up to the eighth reaction cycle. KEY POINTS: • Immobilized microorganism is more efficient than free cell in the caprolactone synthesis. • The reaction times for amino and thiol groups in support were 3 h and 2 h, respectively. • These catalysts showed higher ɛ-caprolactone conversion at higher concentrations.
- Subjects :
- Caproates
Lactones
Geotrichum
Silicon Dioxide
Subjects
Details
- Language :
- English
- ISSN :
- 1432-0614
- Volume :
- 104
- Issue :
- 20
- Database :
- MEDLINE
- Journal :
- Applied microbiology and biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 32902680
- Full Text :
- https://doi.org/10.1007/s00253-020-10875-7